摘要
目的探讨不同临床类型慢性HBV感染患者HBV DNA载量、腺苷脱氨酶(ADA)、miR-181a、自然杀伤(NK)细胞的表达,并分析ADA、miR-181a、NK细胞与HBV DNA载量的关系。方法回顾性选取2017年1月至2020年9月收治的144例HBV感染患者作为研究对象。以疾病类型分为HBV携带者(ASC)、慢性乙型肝炎(CHB)、乙型肝炎肝硬化(LC)以及HBV相关性肝细胞癌(HBV-HCC),其中ASC 37例、CHB 43例、LC 31例、HBV-HCC 33例;另择同时期体检正常的30例健康人作为对照组。收集受试者HBV DNA、ADA、miR-181a及NK细胞等数据,并行分析。结果CHB组HBV DNA载量为(6.07±0.93)copy/mL,显著高于ASC组、LC组、HBV-HCC组的(5.63±0.82)/mL、(5.38±0.73)copy/mL、(4.53±0.42)copy/mL(P<0.05),HBV-HCC组HBV DNA载量水平显著低于ASC组、LC组(P<0.05);HBV-HCC组患者ADA(49.85±7.23)U/L,显著高于ASC组、CHB组、LC组、对照组的(13.28±3.27)U/L、(21.82±4.83)U/L、(35.83±5.59)U/L、(9.43±2.31)U/L(P<0.05),LC组ADA显著高于ASC组、对照组(P<0.05),CHB组ADA显著高于ASC组、对照组(P<0.05),ASC组ADA显著高于对照组(P<0.05);HBV-HCC组NK细胞(4.05±0.73)%,显著低于ASC组、CHB组、LC组、对照组的(9.42±2.51)%、(8.43±1.08)%、(5.89±1.34)%、(11.04±2.87)%(P<0.05),LC组NK细胞显著低于ASC组、CHB组、对照组(P<0.05),CHB组NK细胞显著低于ASC组、对照组(P<0.05),ASC组NK细胞显著低于对照组(P<0.05);CHB组miR-181a相对表达量为(0.71±0.13)2^(-ΔΔCt),显著低于ASC组、LC组、HBV-HCC组、对照组的(0.94±0.08)2^(-ΔΔCt)、(0.94±0.08)2^(-ΔΔCt)、(1.23±0.13)2^(-ΔΔCt)、(0.98±0.04)2^(-ΔΔCt)(P<0.05),HBV-HCC组miR-181a相对表达量显著高于ASC组、LC组、对照组(P<0.05)。在ASC、CHB、LC、HBV-HCC患者中,ADA与HBV DNA载量均呈正相关(r=0.752、0.668、0.413、0.639,P<0.05),NK细胞与HBV DNA载量呈负相关(r=-0.631、-0.473、-0.507、-0.434,P<0.05);miR-181a在CHB患者与HBV DNA载量呈负相关(r=-0.613,P<0.05),在HBV-HCC患者与病毒载量HBV DN
Objective To investigate the changes of hepatitis B virus(HBV)-DNA load,adenosine deaminase(ADA),miR-181a,natural killer(NK)cells in patients with different clinical types of hepatitis B after HBV infection,and the relationship between ADA,miR-181a,NK cells and HBV DNA load.Methods One hundred and forty-four patients with HBV infection admitted to our hospital from January 2017 to September 2020 were retrospectively selected as the research objects.According to disease types,they were divided into HBV carrier(ASC),chronic hepatitis B(CHB),liver cirrhosis(LC)and HBV-associated hepatocellular carcinoma(HBV-HCC)groups,of which 37 cases were ASC,43 cases were CHB,31 cases were LC and 33 cases were HBV-HCC.Another 30 healthy people with normal physical examination during the same period were selected as a control group.Data of HBV DNA load,serum ADA level,plasma miR-181a level,and the percentage of NK cells in peripheral blood mononuclear cells(PBMC)were collected for comparative analysis.Results HBV DNA load in CHB group was(6.07±0.93)copy/mL,which was significantly higher than those of(5.63±0.82)copy/mL,(5.38±0.73)copy/mL and(4.53±0.42)copy/mL in ASC group,LC group and HBV-HCC group,respectively(P<0.05).HBV DNA load in HBV-HCC group was significantly lower than those in ASC group and LC group(P<0.05).The ADA level of HBV-HCC patients was(49.85±7.23)U/L,which was significantly higher than those of(13.28±3.27)U/L,(21.82±4.83)U/L,(35.83±5.59)U/L,(9.43±2.31)U/L in the ASC,CHB group,LC group and control group,respectively(P<0.05).The ADA level of LC group and CHB group were significantly higher than those in the ASC group and control group(P<0.05),and the ADA level in ASC group was significantly higher than that of the control group(P<0.05).The percentage of NK/PBMC cells in HBV-HCC group was(4.05±0.73)%,which was significantly lower than those of(9.42±2.51)%,(8.43±1.08)%,(5.89±1.34)%,(11.04±2.87)%in the ASC group,CHB group,LC group and control group,respectively(P<0.05).The percentage of NK cells in LC gro
作者
李成
张伟
陈曲
张磊
赵秋剑
李耀妮
LI Cheng;ZHANG Wei;CHEN Qu;ZHANG Lei;ZHAO Qiu-jian;LI Yao-ni(Department of Laboratory,Baoji Central Hospital,Shaanxi 721008,China;Infectious Diseases,Xijing Hospital Affiliated to Air Force Military Medical University,Xi′an 710032,China)
出处
《肝脏》
2021年第11期1231-1235,共5页
Chinese Hepatology
基金
宝鸡市卫生和计划生育委员会科研课题(2017-12)。
