摘要
【背景】近年来,马铃薯疮痂病的危害态势逐渐上升,在全国各主要产区均有发生,目前由于缺乏有效的防治手段,已造成了严重的经济损失。生物防治是防治土传病害的有效途径,逐渐成为了研究热点。【目的】筛选对马铃薯疮痂病菌具有较高拮抗效果的菌株,为生防菌剂的开发提供菌种资源,同时也为马铃薯疮痂病的防治奠定理论基础。【方法】采用平板对峙法和牛津杯试验法对分离得到的菌株进行初筛和复筛,并通过形态特征、生理生化特征及16S rRNA基因、gyrB基因序列分析结果对菌株进行鉴定。通过平板对峙法测定菌株的抑菌谱。【结果】获得一株具有明显拮抗效果的菌株BKS104,抑菌直径达到43mm,防效达到85%。其菌落圆形、乳白色、不透明、表面有光泽,边缘整齐,菌体杆状,革兰氏阳性菌。结合16S rRNA基因、gyrB基因的测序结果将其鉴定为贝莱斯芽孢杆菌,并对8种植物病原真菌均具有抑制效果。【结论】菌株BKS104为贝莱斯芽孢杆菌,可以有效抑制马铃薯疮痂病菌的生长,安全性高,具有良好的生防潜力。
[Background] In recent years, the damage of potato scab disease has gradually increased,and it has occurred in all major producing areas across the country. At present, the lack of effective prevention and control methods has caused serious economic losses. Biological control is an effective way to prevent and control soil-borne diseases, and has gradually become a research hotspot.[Objective] Screening strains with high antagonistic effect on Streptomyces scabies will provide strain resources for the development of biocontrol agents and lay a theoretical foundation for the prevention and control of potato scab. [Methods] The isolated strains were preliminarily screened and re-screened by the plate confrontation growth method and the Oxford cup test method, and the strains were identified by morphological characteristics, physiological and biochemical characteristics, and 16 S rRNA and gyrB sequence analysis results. The antifungal spectrum of the strain was determined by the plate confrontation method. [Results] A strain BKS104 with obvious antagonistic effect was obtained, with a antifungal diameter of 43 mm and a control effect of 85%. The colony is round, milky white, opaque, shiny surface, neat edges, rod-shaped bacteria, Gram-positive bacteria. Combined with the sequencing results of 16 S rRNA and gyrB, it was identified as Bacillus velezensis, and it has inhibitory effect on 8 kinds of plant pathogenic fungi. [Conclusion] The strain BKS104 is B.velezensis, which can effectively inhibit the growth of Streptomyces scabies, with high safety and good biocontrol potential.
作者
陈志垚
王鹏
王微
金光辉
台莲梅
郭永霞
孙冬梅
靳学慧
CHEN Zhiyao;WANG Peng;WANG Wei;JIN Guanghui;TAI Lianmei;GUO Yongxia;SUN Dongmei;JIN Xuehui(College of Agronomy,Heilongjiang Bayi Agricultural University,Daqing,Heilongjiang 163319,China;Heilongjiang Provincial Key Laboratory of Crop-Pest Interaction Biology and Ecological Control,Daqing,Heilongjiang 163319,China;College of Life Science and Technology,Heilongjiang Bayi Agricultural University,Daqing,Heilongjiang 163319,China)
出处
《微生物学通报》
CAS
CSCD
北大核心
2021年第11期4145-4155,共11页
Microbiology China
基金
黑龙江省农垦总局重点科研计划(HKKY190207)
黑龙江省应用技术研究与开发计划(GA19B104)
黑龙江八一农垦大学研究生创新科研(YJSCX2019-Y10)。
关键词
马铃薯疮痂病
拮抗细菌
鉴定
potato scab
antagonistic bacteria
identification
