摘要
目的:通过检测进展性脑梗死(PCI)患者血清中内脏脂肪特异性丝氨酸蛋白酶抑制剂(Vaspin)的表达情况,探究其与炎症因子的关系。方法:选取2018年6月至2020年8月本院神经内科收治确诊的105例PCI患者作为PCI组,98例非PCI患者作为NPCI组,同时期健康体检者95例作为对照组。使用全自动生化分析仪检测总胆固醇(TC)、甘油三酯(TG)、低密度脂蛋白胆固醇(LDL-C)、高密度脂蛋白胆固醇(HDL-C)水平;酶联免疫吸附实验(ELISA)检测Vaspin、单核细胞趋化蛋白-1(MCP-1)、白介素17(IL-17)、肿瘤坏死因子α(TNF-α)和降钙素原(PCT)、超敏C反应蛋白(hs-CRP)水平;Vaspin与MCP-1、IL-17、TNF-α、hs-CRP及PCT间相关性采用Pearson相关系数法进行分析;受试者工作特征(ROC)曲线评估Vaspin对PCI的诊断价值;使用Logistic回归分析影响PCI发生的因素。结果:NPCI组和PCI组TC、TG、LDL-C、MCP-1、IL-17、TNF-α、hs-CRP和PCT水平显著高于对照组,而HDL-C和Vaspin水平则显著低于对照组(P<0.05);PCI组TC、TG、LDL-C、MCP-1、IL-17、TNF-α、hs-CRP和PCT水平显著高于NPCI组,而HDL-C和Vaspin水平则显著低于NPCI组(P<0.05);血清中Vaspin水平随疾病进展程度加重而降低(P<0.05);Vaspin与MCP-1、IL-17、TNF-α、hs-CRP、PCT均呈负相关(P<0.05);Vaspin评估PCI发生的ROC曲线下面积为0.914;多因素分析发现低水平Vaspin和HDL-C,高水平MCP-1、IL-17、TNF-α、hs-CRP、PCT、TC、TG和LDL-C均是导致PCI发生的独立危险因素(P<0.05)。结论:PCI患者血清中Vaspin水平与炎症因子密切相关,对疾病诊断及临床监测具有重要参考价值。
Objective:To detect the expression of visceral adipose-specific serine protease inhibitor(Vaspin)in the serum of patients with progressive cerebral infarction(PCI),and to explore its relationship with inflammatory factors.Methods:From June 2018 to August 2020,105 patients with PCI in the department of neurology of our hospital were selected as the PCI group,98 patients with non-progressive cerebral infarction were selected as the NPCI group,and 95 healthy physical examination person during the same period were selected as the control group;the automatic biochemical analyzer was used to detect levels of total cholesterol(TC),triglycerides(TG),lowdensity lipoprotein cholesterol(LDL-C)and high-density lipoprotein cholesterol(HDL-C);ELISA method was used to detect the levels of Vaspin,monocyte chemoattractant protein-1(MCP-1),interleukin-17(IL-17),tumor necrosis factor-α(TNF-α)and procalcitonin(PCT),high sensitivity C-reactive protein(hs-CRP);Pearson correlation coefficient method was used to analyze the correlation between Vaspin and MCP-1,IL-17,TNF-α,hsCRP and PCT;the receiver operating characteristic(ROC)curve was used to assess Vaspin’s diagnostic value for PCI;Logistic regression was used to analyze the factors affecting the occurrence of PCI.Results:Compared with the control group,the levels of TC,TG,LDL-C,MCP-1,IL-17,TNF-α,hs-CRP and PCT in the NPCI group and the PCI group increased significantly,while the levels of HDL-C and Vaspin decreased significantly(P<0.05);compared with the NPCI group,the levels of TC,TG,LDL-C,MCP-1,IL-17,TNF-α,hs-CRP and PCT in the PCI group increased significantly,while the levels of HDL-C and Vaspin decreased significantly(P<0.05);the level of Vaspin in the serum decreased with the degree of progression(P<0.05);Vaspin had a negative correlation with MCP-1,IL-17,TNF-α,hs-CRP,and PCT(P<0.05);the area under the ROC curve of Vaspin in the assessment of PCI was 0.914;multivariate analysis found that low level of Vaspin and HDL-C,high levels of MCP-1,IL-17,TNF-α,hs-CRP,PCT,TC,TG
作者
高倩
王建宇
孟伟建
李静
崔永健
魏琰
GAO Qian;WANG Jianyu;MENG Weijian;LI Jing;CUI Yongjian;WEI Yan(Department of Neurology,Hengshui People's Hospital/Hallison hiternational Peace Hospital,Hengshui 053000,China)
出处
《现代医学》
2021年第9期1006-1011,共6页
Modern Medical Journal
基金
2018年度河北省医学科学研究重点课题计划(20181592)。
关键词
进展性脑梗死
内脏脂肪特异性丝氨酸蛋白酶抑制剂
炎症因子
血清
progressive cerebral infarction
visceral adipose-specific serine protease inhibitor
inflammatory factors
serum
