摘要
桑黄菌是我国传统药食两用的大型稀有真菌,其多糖、黄酮和多酚类物质可以添加到果蔬制品中,能增强营养功效。本文以不同动植物资源作为液态发酵培养基,研究瓦尼桑黄菌液态发酵菌丝体总多酚的最优工艺,首先,利用单因素实验对桑黄菌液态发酵培养基碳源、氮源和生长因子进行筛选,之后采用Plackrtt-Burman实验筛选出对桑黄菌菌丝多酚含量影响最显著的三个因素:小米粉、KH_(2)PO_(4)和接种量;通过中心组合实验设计确定了桑黄菌菌丝多酚含量最优的发酵工艺,即小米粉24.00 g/L、酵母粉10 g/L、桑叶粉10 g/L、KH_(2)PO_(4)1.08 g/L、MgSO_(4)·7H_(2)O 1 g/L、CaCl_(2)1 g/L、pH 6.5、接种量11.64%,在此条件下发酵的桑黄菌菌丝体总多酚得率为2.63 mg/g。验证试验结果表明,此方法菌丝多酚得率的相对误差在±0.5%以内,可以为桑黄菌液态发酵产菌丝多酚提供理论依据。
Sanghuangprous is a large and rare fungus that can be used as both medicine and food in China.Its polysaccharides,flavonoids and polyphenols can be added to fruit and vegetable products to enhance the nutritional effect.In this paper,different animal and plant resouces were used as liquid fermentation medium to study the optimal process of liquid fermentation of total polyphenols from mycelia of Sanghuangprous vaninii.Single factor experiment was used to screen out carbon sources,nitrogen sources and growth factors in the submerged fermentation medium.The three factors having significant effects on total polyphenols production were screened out by using Plackett-Burman design,which was millet flour,KH_(2)PO_(4) and inoculation amount.By central combination experiment design,the optimal fermentation process of polyphenols content was determined that millet flour 24.00 g/L,yeast powder 10 g/L,mulberry leaf powder 10 g/L,KH_(2)PO_(4)1.08 g/L,MgSO_(4)·7H_(2)O 1 g/L,CaCl_(2)1 g/L,pH 6.5,inoculation amount 11.64%.Under these conditions,the total polyphenols content of mycelia was 2.63 mg/g.Verification test results showed that the relative error of mycelial polyphenols yield was within±0.5%,which could provide a theoretical basis for liquid fermentation of mycelial polyphenols by Sanghuangprous.
作者
李学震
郑明珠
马艳蕊
和法涛
刘光鹏
LI Xue-zhen;ZHENG Ming-zhu;MA Yan-rui;HE Fa-tao;LIU Guang-peng(Jilin Agricultural University,Changchun 130118,China;Jinan Fruit Research Institute,All China Federation of Supply&Marketing Co-operatives,Jinan 250220,China)
出处
《中国果菜》
2021年第11期13-19,共7页
China Fruit & Vegetable
基金
山东省农业科技基金(2019LY007-03)。
关键词
瓦尼桑黄菌
多酚
响应曲面
培养基优化
Sanghuangprous
polyphenol
response surface
medium optimization
