摘要
通过估算湿加松亲本间SNP位点遗传距离,以有效预测杂交后代的树高、胸径、材积杂种优势,为分子辅助交配设计育种提供参考。利用SLAF-seq技术对131个湿加松亲本种质资源进行测序,获得有效的SNP标记;基于SNP位点信息,利用MEGA5.0软件估算13个湿加松亲本间遗传距离,并进行聚类分析,同时利用SPSS软件分析13个杂交组合生长性状杂种优势与SNP遗传距离的相关性。SLAF-seq共获得53952个多态性SLAF标签,96736个有效SNP标记;湿加松亲本间遗传距离介于0.4251~0.4906,亲本间SNP遗传距离与树高、胸径、材积生长性状杂种优势相关系数分别为0.680、0.648、0.624,均达到显著正相关水平。SLAF-seq技术可提供海量SNP位点,根据海量SNP位点信息可估算湿加松亲本间遗传距离,以有效预测树高、胸径、材积杂种优势。
Using SNP loci to estimate the genetic distance between the parents of Pinus elliottii×P.caribaea,and effectively predicting the heterosis of tree height,diameter at breast height(DBH)and timber volume in their hybrids,which provides a reference for molecular marker-assisted mating design and breeding.Using SLAF-seq technology to sequence and analyze the DNAs of 131 germplasm resources from the parents of P.elliottii×P.caribaea,and valid SNP markers were obtained.Based on these SNP loci,the genetic distances between 13 parents of P.elliottii×P.caribaea were estimated by using MEGA 5.0 software,and cluster analysis were performed.The heterosis of the growth traits and the correlation between heterosis and SNP genetic distance of the 13 cross combinations were analyzed and calculated by using SPSS software.A total of 53952 polymorphic SLAF tags and 96736 valid SNP markers were obtained by SLAP-seq.The genetic distance between the parents of P.elliottii×P.caribaea was arranged from 0.4251-0.4906,and the correlation coefficients between SNP genetic distance of the parents and heterosis of their hybrids in tree height,DBH and timber volume were 0.680,0.648 and 0.624,respectively.All three correlation coefficients reached a significantly positive correlation level.Utilizing SLAF-seq technology can obtain massive SNP loci.Based on these SNPs,the genetic distance between parents of P.elliottii×P.caribaea can be estimated,and the heterosis of tree height,DBH and timber volume in the hybrids can be effectively predicted.
作者
李义良
赵奋成
刘阳
钟岁英
林昌明
谭志强
李福明
廖仿炎
吴惠姗
郭文冰
王哲
LI Yi-Liang;ZHAO Fen-Cheng;LIU Yang;ZHONG Sui-Ying;LIN Chang-Ming;TAN Zhi-Qiang;LI Fu-Ming;LIAO Fang-Yan;WU Hui-Shan;GUO Wen-Bin;WANG Zhe(Guangdong Provincial Key Laboratory of Silviculture,Protection and Utilization,Guangzhou 510520;Guangdong Academy of Forestry,Guangzhou 510520;Taishan Hongling Seed Orchard,Taishan 529200)
出处
《植物研究》
CAS
CSCD
北大核心
2021年第5期738-743,共6页
Bulletin of Botanical Research
基金
广东省林业科技创新项目(2019KJCX019)。
关键词
湿加松
SNP标记
遗传距离
杂种优势
Pinus elliottii×P.caribaea
SNP marker
genetic distance
heterosis
