摘要
目的:探讨青藤碱对HPV阳性宫颈癌SiHa细胞增殖和侵袭的影响。方法:体外培养SiHa细胞,在细胞培养液中分别加入0.2、0.4和0.8 mmol/L的青藤碱,阴性对照组细胞未加青藤碱,24和48 h后采用CCK-8法检测细胞活力。细胞培养液加入0.2mmol/L的青藤碱,24 h后采用免疫荧光法检测Ki67阳性细胞数;Transwell实验检测侵袭细胞数;Western blot检测侵袭相关蛋白MMP-2和MMP-9的蛋白表达水平。结果:与阴性对照组相比,0.2、0.4和0.8 mmol/L青藤碱作用SiHa细胞24和48 h后,SiHa细胞的增殖率均明显降低(P<0.01);青藤碱处理组SiHa细胞中Ki67阳性细胞数减少(P<0.01),发生侵袭的SiHa细胞数减少(P<0.05),侵袭相关蛋白MMP-2和MMP-9蛋白的表达均降低(P<0.01)。结论:在体外,青藤碱可以抑制体外培养的HPV阳性宫颈癌SiHa细胞的增殖和侵袭。
OBJECTIVE:To investigate effects of sinomenine on proliferation and invasion of HPV-positive cervical cancer cells.METHODS:In the negative control group,0 mmol/L sinomenine was added tothe SiHa cell culture medium.In the experimental group,0.2,0.4 and 0.8 mmol/L sinomenine were added.CCK-8 was used to detect cell viability after 24 and 48 h.In the sinomenine group,0.2 mmol/L sinomeninewas added to SiHa cell culture medium,and in the negative control group,0 mmol/L sinomenine was addedto SiHa cell culture medium.After 24 hours,the number of Ki67-positive cells was detected byimmunofluorescence.Transwell experiment was used to detect the number of invaded cells.Western blot wasused to detect protein expression levels of invasion-related proteins MMP-2 and MMP-9.RESULTS:After0.2,0.4 and 0.8 mmol/L of sinomenine treatment for 24 and 48 h,proliferation rates of the SiHa cells weresignificantly lower than that of the control group(P<0.01).Compared with the negative control group,thenumbers of Ki67-positive cells in the sinomenine group were significantly reduced(P<0.01).Compared withthe negative control group,invasions in the sinomenine group were significantly reduced(P<0.05).Comparedwith the control group,expressions of the invasion-related proteins MMP-2 and MMP-9 in the sinomeninegroup were significantly reduced(P<0.01).CONCLUSION:Sinomenine inhibited proliferation and invasion ofHPV-positive cervical cancer cells.
作者
王晓舟
李洪石
于溪
魏宁
王梓瑛
赫丽杰
WANG Xiaozhou;LI Hongshi;YU Xi;WEI Ning;WANG Ziying;HE Lijie(Department of Oncology/Radiotherapy,the Peoples Hospital of Liaoning Province,Shenyang 110016,Liaoning,China)
出处
《癌变．畸变．突变》
CAS
2021年第6期442-445,共4页
Carcinogenesis,Teratogenesis & Mutagenesis
基金
沈阳市中青年科技创新人才支持计划项目(RC180334)。
