摘要
背景:骨关节炎的病理进程中有多种因素共同作用,包括滑膜炎症、软骨丢失、软骨下骨损害、骨质增生等,其具体机制尚未明确。Micro CT是一种非破坏性成像工具,可以形成样本的高分辨率3D图像,实现骨和软骨的相关结构和功能的研究、疾病进展和治疗的定量评估及对比增强软组织成像。通过micro CT扫描骨关节炎动物模型,可以明确在骨关节炎病程中关节、关节软骨、软骨下骨、滑膜、血管等组织的病理变化,有利于骨关节炎病理进展的研究。目的:归纳总结micro CT在骨关节炎动物模型的关节、软骨、软骨下骨、滑膜、骨内血管的成像方法及研究展望,为骨关节炎的病理机制变化研究提供新的方法。方法:通过检索中国知网、Web of Science、Embase、PubMed、万方等数据库,英文关键词为"Osteoarthritis,micro CT,Pathology"等,中文关键词为"骨关节炎,micro CT,病理"等,选取相关性强,发表年限较新的合格文献进行综述。结果与结论:(1)Micro CT在关节、软骨、软骨下骨、骨内血管都有较好的成像表现,成像方法成熟,改变传统病理切片破坏组织整体性、步骤繁琐的缺点。(2)Micro CT在滑膜的成像方面尚未有文献报道,但在如脂肪、肿瘤等软组织的成像中有着不错的表现,对滑膜的成像在未来有研究前景。(3)Micro CT可以对标本进行三维立体重建,打破常规二维病理切片的局限性,为骨关节炎的病理机制研究提供新的模式。
BACKGROUND:There are many factors in the pathological process of osteoarthritis,including synovial inflammation,cartilage loss,subchondral bone damage,bone hyperplasia and so on.The specific mechanism is not yet clear.Micro CT is a non-destructive imaging tool,which can form high-resolution 3 D images of samples,and realize the research of related structures and functions of bone and cartilage,quantitative evaluation of disease progression and treatment,and contrast-enhanced soft tissue imaging.Micro CT scanning of osteoarthritis animal model can clarify the pathological changes of joints,articular cartilage,subchondral bone,synovium,blood vessels and other tissues in the course of osteoarthritis,which is conducive to the study of the pathological progress of osteoarthritis.OBJECTIVE:To summarize the imaging methods and research prospects of micro CT in joints,cartilage,subchondral bone,synovium and intraosseous vessels of osteoarthritis animal model,so as to provide a new method for the study of the pathological mechanism of osteoarthritis.METHODS:By searching the databases of CNKI,Web of Science,Embase,PubMed and Wanfang,the Chinese and English keywords were"osteoarthritis,micro CT,pathology".The qualified articles with strong correlation and relatively new publication years were selected for review.RESULTS AND CONCLUSION:(1)Micro CT has good imaging performance in joints,cartilage,subchondral bone,and intraosseous vessels.The imaging method is mature,which changes the shortcomings of traditional pathological sections on destroying tissue integrity and cumbersome steps.(2)Micro CT has not been reported in the literature on the imaging of synovium,but it has a good performance in the imaging of soft tissues,such as fat and tumor.The imaging of synovium has a research prospect in the future.(3)Micro CT can carry out three-dimensional reconstruction of specimens,break through the limitations of conventional two-dimensional pathological sections,and provide a new model for the study of the pathological mechanism of
作者
杜龙龙
袁普卫
杨威
李雪枫
高启萌
Du Longlong;Yuan Puwei;Yang Wei;Li Xuefeng;Gao Qimeng(Shaanxi University of Chinese Medicine,Xianyang 712046,Shaanxi Province,China;Guangzhou University of Chinese Medicine,Guangzhou 510006,Guangdong Province,China)
出处
《中国组织工程研究》
CAS
北大核心
2022年第12期1931-1936,共6页
Chinese Journal of Tissue Engineering Research
基金
长安医学关中李氏骨伤学术流派传承工作室建设项目(陕中医药发[2018]40号),项目负责人:袁普卫
陕西省骨退行性疾病中西医结合防治重点研究室建设项目(陕中医药发[2018]32号),项目负责人:袁普卫
咸阳市重点实验室建设项目(2019k01-53),项目负责人:袁普卫。
关键词
骨关节炎
Micro
CT
动物模型
病理
软骨
软骨下骨
骨内血管
综述
osteoarthritis
micro CT
animal model
pathology
cartilage
subchondral bone
intraosseous blood vessel
review
