摘要
目的在SD雄性大鼠建立肝胆管炎症模型的基础上,探究大黄灵仙颗粒干预肝内胆管结石形成的作用机制。方法将48只SD雄性大鼠随机分为正常组、脂多糖模型组、熊去氧胆酸对照组、大黄灵仙颗粒实验组,每组12只。正常组予常规饲养,模型组、对照组、实验组在胆总管一次性注射1.25mg/kg脂多糖诱导胆管炎症形成,同时对照组和实验组分别连续进行药物干预7天。取血液进行ELISA检测,取肝脏标本进行HE染色和RT-PCR检测。结果与其他三组相比,模型组肝脏组织病理形态提示炎症浸润程度明显;各组大鼠IL-6、IL-8、TNF-α、NF-κB蛋白及mRNA表达总体差异有统计学意义(P<0.01);与正常组相比较,模型组和对照组IL-6、IL-8、TNF-α、NF-κB蛋白及mRNA表达差异均明显增高(P<0.01);与正常组相比较,实验组IL-6、TNF-α蛋白表达无明显差异(P>0.05);与正常组相比较,实验组IL-6、IL-8、TNF-α、NF-κB的mRNA表达无明显差异(P>0.05)。结论 LPS胆管注射可以诱导大鼠胆管细胞细胞释放炎症因子引起急性胆管炎,大黄灵仙颗粒具有减少炎症因子释放,从而达到阻断肝内胆管结石形成。
Objective To explore the mechanism of Dahuang Lingxian Granule on the formation of hepatolithiasis in male SD rats.Methods 48 male SD rats were randomly divided into normal group,lipopolysaccharide model group,ursodeoxycholic acid control group and Dahuang Lingxian granule experimental group,with 12 rats in each group.The model group,the control group and the experimental group were injected with 1.25 mg/kg lipopolysaccharide in the common bile duct to induce bile duct inflammation.At the same time,the control group and the experimental group were given drug intervention for 7 days.Blood samples were collected for ELISA detection,and liver samples were detected by HE staining and RT-PCR.Results Compared with the other three groups,the pathological morphology of liver tissue in the model group showed obvious degree of inflammatory infiltration;the protein and mRNA expression of IL-6,IL-8,TNF-α,NF-κB in each group were significantly different(P<0.01);compared with the normal group,the expression of IL-6,IL-8,TNF-α,NF-κB protein and mRNA in the model group and the control group were significantly increased(P<0.01);compared with the normal group,the expression of IL-6,IL-8,TNF-α,NF-κB protein and mRNA were significantly increased(P<0.01).There was no significant difference in the expression of IL-6 and TNF-αin the experimental group(P>0.05);compared with the normal group,the mRNA expression of IL-6,IL-8,TNF-α,NF-κB in the experimental group had no significant difference(P>0.05).Conclusion LPS bile duct injection can induce rat bile duct cells to release inflammatory factors and cause acute cholangitis.Dahuang Lingxian granule can reduce the release of inflammatory factors,thus blocking the formation of intrahepatic bile duct stones.
作者
许彦
王兵
唐乾利
黄丽芳
曾鸿孟
黄汉基
XU Yan;WANG Bing;TANG Qian-li;HUANG Li-fang;ZENG Hong-meng;HUANG Han-ji(First Affiliated Hospital of Guangxi University of Chinese Medicine,Nanning,Guangxi,530023,China;Youjiang Medical University for Nationalities,Baise,Guangxi 533000,China;Guangxi International Zhuang Medical Hospital,Nanning,Guangxi,530201,China)
出处
《时珍国医国药》
CAS
CSCD
北大核心
2021年第9期2074-2078,共5页
Lishizhen Medicine and Materia Medica Research
基金
国家自然科学基金(81804099,81860851)
广西科学研究与技术开发计划项目(重点研发计划)(桂科AB17195071)
广西科技计划项目“广西肝胆疾病临床医学研究中心”(桂科AD17129025-27)
广西自然科学基金(2018GXNSFDA281017,2020JJB140118)
广西中医药大学研究生教育创新计划资助项目(YCSY2020100)。
关键词
胆管炎症
信号通路
炎症因子
大黄灵仙颗粒
Cholangitis
Signaling pathways
Lnflammatory factors
Dahuang Lingxian granules
