摘要
为获知棘头梅童鱼亲鱼性腺发育后期脂类及脂肪酸蓄积特点,采用内标定量法,对性腺发育至Ⅳ期的雌性棘头梅童鱼亲鱼肌肉、肝脏和卵巢组织中的脂肪及脂肪酸含量进行了测定,并比较分析了3种组织中的脂肪酸组成。结果表明:雌性棘头梅童鱼亲鱼卵巢、肝脏和肌肉中脂肪的质量分数分别为(4.84±0.05)%、(2.66±0.05)%和(0.56±0.05)%,不同组织间具有显著性差异(P<0.05)。雌性棘头梅童鱼亲鱼的卵巢、肌肉和肝脏中分别检出29种脂肪酸,含量最高的饱和脂肪酸依次为C16∶0、C18∶0和C14∶0;含量最高的单不饱和脂肪酸依次为C16∶1、C18∶1n9c和C20∶1;含量最高的多不饱和脂肪酸依次为C22∶6n3(DHA)、C20∶5n3(EPA)和C20∶4n6(ARA)。从相对含量来看,肌肉中饱和脂肪酸的质量分数为(58.22±0.24)%,显著高于肝脏(52.71±0.24)%和卵巢(36.42±0.24)%(P<0.05);卵巢中单不饱和脂肪酸的质量分数为(23.78±0.09)%,显著高于肌肉(16.15±0.07)%和肝脏(10.79±0.05)%(P<0.05);卵巢中多不饱和脂肪酸质量分数为(39.79±0.19)%,显著高于肝脏(36.49±0.21)%和肌肉(25.63±0.19)%(P<0.05)。肌肉、肝脏和卵巢中EPA和DHA的总量依次升高,质量分数分别为(19.34±0.15)%、(26.59±0.16)%和(27.78±0.13)%。卵巢、肝脏和肌肉组织中的DHA/EPA分别为1.95、2.28和1.89,n-3系和n-6系多不饱和脂肪酸的比值分别为3.29、3.81和6.25。
This paper studied the lipid content and fatty acid composition of muscle,liver and ovary of female adult Collichthys lucidus broodstocks in StageⅣof ovarian maturity.The results showed that the lipid content in ovary,muscle and liver were(4.84±0.05)%,(0.56±0.05)%and(2.66±0.05)%with significant differences(P<0.05).Totally 29 fatty acids were detected in the ovary,muscle and liver of C.lucidus broodstocks,and C16∶0,C18∶0 and C14∶0 were the main saturated fatty acids(SFA);C16∶1,C18∶1n9c and C20∶1 were the main monounsaturated fatty acids(MUFA);C22∶6n3(DHA),C20∶5n3(EPA)and C20∶4n6(ARA)were the main polyunsaturated fatty acids(PUFA).In terms of percentage content,the percentage of SFA in muscle was significantly higher than that in liver and ovary(P<0.05).The percentage of MUFA was the highest in the ovary,followed by the content in the muscle,and the lowest in the liver(P<0.05).The percentage of PUFA was the highest in the ovary,followed by the content in the liver,and the lowest content in the muscle(P<0.05).The percentage of EPA and DHA in muscle,liver and ovary were(19.34±0.15)%,(26.59±0.16)%and(27.78±0.13)%with an increasing trend.The values of DHA/EPA in ovary,liver and muscle tissue were 1.95,2.28 and 1.89,and the ratios of n-3 and n-6 polyunsaturated fatty acids were 3.29,3.81 and 6.25,respectively.
作者
曹平
宋炜
陈佳
谢正丽
王鲁民
CAO Ping;SONG Wei;CHEN Jia;XIE Zhengli;WANG Lumin(Key Laboratory of Oceanic and Polar Fisheries,Ministry of Agriculture and Rural Affairs,East China Sea Fisheries Research Institute,Chinese Academy of Fishery Sciences,Shanghai 200090,China;College of Fisheries and Life Sciences,Shanghai Ocean University,Shanghai 201306,China;State Key Laboratory of Large Yellow Croaker Breeding,Fuding Seagull Fishing Food Co.Ltd.,Ningde 352103,China;Key Laboratory of Fishery Equipment and Engineering Technology,Ministry of Agriculture and Rural Affairs,Fishery Machinery and Instrument Research Institute,Chinese Academy of Fishery Sciences,Shanghai 200092,China)
出处
《水产科技情报》
2021年第6期301-306,共6页
Fisheries Science & Technology Information
基金
中央级公益性科研院所基本科研业务费专项资金(东2019M02)
浙江省重点研发项目(2020C02015)
科技部国家重点研发计划--蓝色粮仓科技创新专项(2020YFD0900805)
现代农业产业技术体系专项(CARS-47-G25)
中国水产科学研究院科技创新团队项目(2020TD14)。
关键词
棘头梅童鱼
卵巢
肌肉
肝脏
脂肪酸
Collichthys lucidus
ovary
muscle
liver
fatty acid
