摘要
目的:探讨L-苏糖酸镁在高磷诱导大鼠慢性肾功能衰竭(CRF)血管钙化(VC)模型中的作用及其机制。方法:采用随机数表法将56只雄性SD大鼠分为6组,即对照组(9只)、高磷饮食组(10只)、CRF模型组(10只)、CRF模型+低镁组(10只,模型+低镁组)、CRF模型+L-苏糖酸镁(3.67 g·kg^(-1)·d^(-1))组(9只,模型+高镁组)和CRF模型+醋酸钙组(8只,模型+高钙组)。采用硫酸腺嘌呤加高磷饮食建立大鼠CRF模型。造模成功后测量6组大鼠血清肌酐、尿素氮、磷、钙、镁等水平,进行肾脏苏木精-伊红(HE)染色,采用逆转录定量聚合酶链反应(qRT-PCR)方法检测大鼠主动脉VC标志分子核心结合因子α1(Cbfα1)mRNA的表达情况,行Von Kossa染色并测定腹主动脉钙水平。结果:建模第7周,CRF模型组大鼠血清肌酐、尿素氮及磷水平均高于高磷饮食组,差异有统计学意义(t=-10.299,t=-26.284,t=-2.938;P<0.05)。干预第8周,模型+低镁组大鼠血清肌酐水平高于其余5组,差异有统计学意义(F=95.43,P<0.05),模型+高钙组钙水平高于其余5组,差异有统计学意义(F=2.709,P<0.05)。6组大鼠血清镁水平的比较,差异有统计学意义(F=9.403,P<0.05)。模型+高钙组大鼠胸主动脉Cbfα1 mRNA表达量高于其余5组,差异有统计学意义(F=14.406,P<0.05)。结论:L-苏糖酸镁抑制高磷诱导的CRF大鼠VC,其可能机制是L-苏糖酸镁通过抑制高磷诱导慢性肾衰竭大鼠血管平滑肌细胞表型转化来实现。
Objective:To investigate the effect and mechanism of magnesium L-threglycoate on high phosphorusinduced vascular calcification in rats with chronic renal failure(CRF).Methods:56 male SD rats were divided into 6 groups by random number table method,which included control group(n=9),high-phosphorus diet group(n=10),CRF model group(n=10),CRF model+low-magnesium group(n=10),CRF model+L-threosaccharide magnesium(3.67g·kg-1·d-1)group(n=9,model+high magnesium group)and CRF model+calcium acetate group(n=8 cases,model+high calcium group).Rats with CRF model was established by adenine sulfate combined with high phosphorus diet.After successful modeling,the levels of serum creatinine,urea nitrogen,phosphorus,calcium and magnesium of 6 groups were measured.Hematoxylin-eosin(HE)staining was performed on the kidneys,and the mRNA expression of core binding factorα1(Cbfα1)of the mark molecule of aortic vascular calcification of rats were detected by adopting quantitative reverse transcription polymerase chain reaction(QRT-PCR).Von Kossa staining was used to determine calcium levels in abdominal aorta.Results:At the 7th week of modeling,the levels of serum creatinine,urea nitrogen and phosphorus in CRF model group were significantly higher than those in high-phosphorus diet group(t=-10.299,t=-26.284,t=-2.938,P<0.05).At the 8th week of intervention,serum creatinine level in model+low magnesium group was significantly higher than that in the other 5 groups(F=95.43,P<0.05),and calcium level in model+high calcium group was significantly higher than that in the other 5 groups(F=2.709,P<0.05),respectively.There was statistical significance in serum magnesium level among 6 groups(F=9.403,P<0.05).The expression level of Cbfα1 mRNA in thoracic aorta of rats in model+high calcium group was significantly higher than that in the other five groups,and the difference of that among these groups was statistically significant(F=14.406,P<0.05).Conclusion:Magnesium L-threglycoate can inhibit vascular calcification in rats with CRF which were i
作者
董丽明
李寅辉
米克热衣·艾孜买提
李莉
DONG Li-ming;LI Yan-hui;MIKEREYI Aizi-maiti(Department of Clinical Nutrition,The First Affiliated Hospital of Xingjiang Medical University,Urumqi 830000,China)
出处
《中国医学装备》
2021年第11期170-177,共8页
China Medical Equipment
基金
新疆维吾尔自治区科学技术课题(2018D01C206)“碳酸镁干预高磷诱导慢性肾衰竭大鼠血管钙化模型及其相关机制研究”。
