摘要
针对传统连续流光寻址电位传感分析系统存在死体积、样品分析液消耗量大的问题,本文提出了一种基于光寻址电位传感器的液滴分析芯片。液滴分析芯片采用2个注射微泵,在T型微流路中形成了1μL的样品液滴。并对样品液滴的I/t特性进行了研究,当样品液滴经过LAPS检测位点时,光电流突然增加至0.1μA左右;最后测量了pH=5,7,9的缓冲液液滴的I/V特性曲线,得出液滴分析芯片的灵敏度为55 mV/pH,与Nernst理论值基本一致。
Aiming at the problem of large sample consumption in traditional continuous stream light-addressable potentiometric sensor analysis system,this paper proposed a droplet analysis chip based on light-addressable potentiometric sensor.The analysis system used two injection micropumps to form sample droplets in the T-shaped micro flow path.Then the I/t characteristics of the sample droplets were studied.When the sample droplets passed the LAPS detection site,the photocurrent suddenly increased to about 0.1μA.Finally,the pH=5,7,9 buffer droplets were measured.The I/V characteristic curve shows that the sensitivity of the droplet analysis chip is 55 mV/pH,which is in line with the Nernst theoretical value.
作者
李学亮
刘诗斌
LI Xue-liang;LIU Shi-bin(School of Mechanical and Electrical Engineering,Zhoukou Normal University,Zhoukou 466001,China;College of Electronics and Information,Northwestern Polytechnical University,Xi an 710072,China)
出处
《仪表技术与传感器》
CSCD
北大核心
2021年第11期7-9,22,共4页
Instrument Technique and Sensor
基金
国家自然科学基金项目(61873209)。
关键词
光寻址电位传感器
液滴
微流控芯片
light-addressable potentiometric sensor
droplet
microfluidic chip