摘要
目的分析宫颈腺癌及鳞癌中人乳头瘤病毒(HPV)高危亚型分布和相关蛋白表达特征。方法选取2018年1月至2020年2月于江门市中心医院确诊的宫颈癌合并高危型HPV感染患者120例为研究对象,采用第二代杂交捕获技术(HC2)法分析所有患者HPV具体亚型,免疫组化法检测HPV E6、E7、P53和Rb蛋白的表达情况,分析宫颈腺癌和鳞癌中不同HPV高危亚型的表达差异及E6、E7、P53、Rb蛋白表达的差异,分析HPV高危亚型与E6、E7、P53、Rb蛋白表达的相关性。结果120例宫颈癌患者中,腺癌34例,鳞癌86例。宫颈腺癌的HPV 18亚型占比高于鳞癌,HPV 16亚型占比低于鳞癌,且宫颈腺癌的多重感染率高于鳞癌,差异有统计学意义(P<0.05)。宫颈腺癌和鳞癌的E6蛋白表达比较,差异无统计学意义(P>0.05);宫颈腺癌E7蛋白表达高于宫颈鳞癌,P53和Rb蛋白表达低于宫颈鳞癌,差异有统计学意义(P<0.05)。宫颈癌患者HPV 16亚型、HPV 18亚型与E6、E7、P53和Rb蛋白阳性表达均呈正相关(P<0.05)。结论宫颈鳞癌高危HPV亚型以HPV 16型为主,宫颈腺癌高危HPV亚型以HPV 18型为主,且宫颈鳞癌和腺癌E7、P53和Rb蛋白阳性表达存在较大差异,宫颈腺癌多重感染率较高和E6、E7的共表达率较高可能是影响预后不良的重要因素,值得进一步研究。
Objective To analyze the distribution and expression characteristics of high risk human papillomavirus(HPV)subtypes in cervical adenocarcinoma and squamous cell carcinoma.Methods A total of 120 patients with cervical cancer complicated with high-risk HPV infection diagnosed in Jiangmen Central Hospital from January 2018 to February 2020 were selected as the research subjects.HPV subtypes were analyzed by hybrid captune(HC2)method,and the expression of HPV E6,E7,P53 and Rb proteins were detected by immunohistochemical method,the expression differences of HPV high-risk subtypes and E6,E7,P53,Rb protein in cervical adenocarcinoma and squamous cell carcinoma were analyzed,and the correlation between HPV high-risk subtypes and E6,E7,P53,Rb protein expression were analyzed.Results Among the 120 cases of cervical cancer,34 cases were adenocarcinoma and 86 cases were squamous cell carcinoma.The proportion of HPV 18 subtype in cervical adenocarcinoma was higher than that in squamous carcinoma,and the proportion of HPV 16 subtype was lower than that in squamous carcinoma,and the multiple infection rate in cervical adenocarcinoma was higher than that in squamous carcinoma,the differences were statistically significant(P<0.05).There was no significant difference in E6 protein expression between cervical adenocarcinoma and squamous cell carcinoma(P>0.05).The expression of E7 protein in cervical adenocarcinoma was higher than that in cervical squamous cell carcinoma,while the expression of P53 and Rb protein was lower than that in cervical squamous cell carcinoma,the differences were statistically significant(P<0.05).HPV 16 subtype,HPV 18 subtype and positive expression of E6,E7,P53 and Rb protein in cervical cancer patients were positively correlated(P<0.05).Conclusion HPV 16 is the main high-risk HPV subtype of cervical squamous cell carcinoma,and HPV 18 is the main high-risk HPV subtype of cervical adenocarcinoma.There are large differences in the positive expression of E7,P53 and Rb protein between cervical squamous cell c
作者
陈晨
王浩渊
CHEN Chen;WANG Hao-yuan(Department of Oncology,Jiangmen Central Hospital,Guangdong Province,Jiangmen529030,China;Department of Critical Care Medicine,Jiangmen Central Hospital,Guangdong Province,Jiangmen529030,China)
出处
《中国当代医药》
CAS
2021年第32期19-22,共4页
China Modern Medicine
基金
广东省江门市医疗卫生领域科技计划项目(2018A012)。
关键词
宫颈癌
病理类型
人乳头瘤病毒
高危亚型
蛋白表达
特征
Cervical cancer
Pathological types
Human papillomavirus virus
High-risk subtypes
Protein expression
Characteristics