摘要
为研究姜曲海猪FoxO3基因组织表达和遗传多态性与肉质性状的关联性,采用PCR-SSP分子标记技术,分析了112头姜曲海猪FoxO3基因第二外显子的遗传变异情况,利用实时荧光定量PCR检测姜曲海猪心脏、肝脏、脾脏、肾脏、腹脂、皮下脂肪、背最长肌、比目鱼肌、腓肠肌和半腱肌中FoxO3基因的表达量。姜曲海猪FoxO3基因第二外显子500 bp位点存在多态性,得到TT、CC及TC 3种基因型,T基因频率为0.424,表现为中度多态(PIC=0.369);试验猪群的基因频率和基因型频率都处于哈迪温伯格平衡状态(P>0.05);分析不同基因型对肉质性状的影响,FoxO3基因第二外显子多态性与猪肌内脂肪含量、剪切力性状关联显著。FoxO3基因在姜曲海猪10个组织中均有表达,FoxO3基因与肌纤维直径和肌内脂肪含量呈负相关,与肌纤维密度和剪切力呈正相关,但相关性均不显著(P>0.05)。FoxO3基因与腓肠肌肌原纤维直径相关系数分别为-0.937,与背最长肌肌节长度的相关系数为-0.874。综上,FoxO3基因对姜曲海猪肉质性状发育有一定的影响,可作为肉质性状候选基因进行深入研究。
To define the expression and polymorphism of FoxO3 gene and their correlations with meatquality in Jiangquhai pig,PCR‑SSP molecular marker technique was used in the study and the genetic variation of FoxO3 gene exon 2 in 112 Jiangquhai pigs was analyzed,and then the expression of FoxO3 in heart,liver,spleen,kidney,abdominal fat,subcutaneous fat,longissimus dorsi,soleus,gastrocnemius and semitendinosus in Jiangquhai pig was detected by RT‑qPCR.The results indicated that there was polymorphism in exon 2 with three genotypes(TT/TC/CC),and the frequency of allele T was 0.424,showing middle polymorphism(PIC=0.369).The gene frequencies and genotype frequencies of pigs tested reached the Hardy‑Weinberg equilibrium(P>0.05).Analysis of the effect of different gengtypes on meat quality showed that there was significant correlation between polymorphism of FoxO3 gene and intramuscular fat(IMF)content,shear stress.FoxO3 gene was expressed in all ten tissues of Jiangquhai pig.FoxO3 gene was negatively correlated with muscle fiber diameter and intramuscular fat content,but positively correlated with muscle fiber density and shear force.The correlation coefficient between FoxO3 gene and gastrocnemius myofibril diameter was-0.937,and the correlation coefficient between FoxO3 gene and longissimus dorsi was-0.874.The results showed that FoxO3 gene had a certain effect on the development of meat quality traits in Jiangquhai pig,which could be used as a candidate gene affecting the meat quality in Jiangquhai pig.
作者
朱淑斌
倪黎纲
陈章言
张君胜
周春宝
赵秀玲
ZHU Shubin;NI Ligang;CHEN Zhangyan;ZHANG Junsheng;ZHOU Chunbao;ZHAO Xiuling(Jiangsu Animal Husbandry and Veterinary College,Taizhou 225300,China;Jiangquhai Pig Breed Conservation Farm,Taizhou 225300,China)
出处
《河南农业科学》
北大核心
2021年第10期124-131,共8页
Journal of Henan Agricultural Sciences
基金
江苏农牧科技职业学院课题(NSF201802)
江苏农牧科技职业学院横向项目(2020321201000092)
2020年江苏省大学生创新创业训练计划项目(202012806017Y)。
