摘要
为揭示非洲菊肉桂醇脱氢酶基因(GjCAD)的序列特性及其在非生物胁迫下的表达模式,利用反转录PCR(RTPCR)从非洲菊品种‘玲珑’中克隆获得两个GjCADs(GjCAD3和GjCAD5),分析它们及其编码蛋白序列特性,并利用实时荧光定量PCR(qRT-PCR)技术研究它们在不同组织器官(根、叶和叶柄)和不同逆境处理(4℃低温、PEG模拟干旱、水杨酸和NaCl处理)下的表达情况.结果显示:GjCAD3和GjCAD5编码序列(CDS)长度分别为1089 bp和1077 bp,它们编码的蛋白均不含信号肽和跨膜结构域,其中GjCAD3属于MDR超家族,为亲水蛋白;GjCAD5属于PLN02514超家族,为疏水蛋白.qRT-PCR结果显示,GjCAD3和GjCAD5均在根中表达量最高,其次为叶柄,叶最低.低温处理下,GjCAD3除在处理24 h后的表达量显著低于对照外,其余时间点均显著高于对照,而GjCAD5的表达则受到显著抑制;干旱处理下,GjCAD3的表达受到显著抑制,而GjCAD5在处理1 h和12 h的表达量显著高于对照,其余时间点与对照相差不大;SA处理下,GjCAD3的表达受到显著抑制,GjCAD5也仅在处理24 h的表达量显著高于对照;盐胁迫处理下,GjCAD3受到显著抑制,而GjCAD5受到显著诱导.本研究表明GjCAD3和GjCAD5参与非洲菊非生物胁迫应答,但二者表达模式差异较大.该结果可为揭示非洲菊CAD基因的功能奠定基础.
To reveal the sequence characteristics and expression patterns of the cinnamyl alcohol dehydrogenase gene(GjCAD)under different abiotic stress treatments in gerbera.Using reverse transcription PCR(RT-PCR),two GjCADs(GjCAD3 and GjCAD5)genes were successfully cloned from gerbera jamesonii‘Linglong’.A series of bioinformatics software was used to characterize their nucleotide and protein sequences.Quantitative real-time quantitative PCR(qRT-PCR)was used to show their expression patterns in different tissues and organs(including roots,petioles,and leaves)and under different abiotic stresses(including 4℃low temperature,PEG mimicked drought,salicylic acid(SA),and NaCl treatments).The coding sequences(CDS)of GjCAD3 and GjCAD5 were 1089 bp and 1077 bp,respectively.Both their encoded proteins do not contain signal peptides or transmembrane structures.GjCAD3 is a hydrophilic protein belonging to the MDR superfamily,while GjCAD5 is a hydrophobic protein belonging to the PLN02514 superfamily.qRT-PCR results showed that both GjCAD3 and GjCAD5 expressed the highest expression in roots,followed by petioles and leaves.Under low-temperature treatment,the expression of GjCAD3 was significantly induced at all time points except at 24 h(significantly lower than control).In contrast,the expression of GjCAD5 was significantly inhibited at all time points.Under drought treatment,GjCAD3 was significantly suppressed,but GjCAD5 expression at 1 h and 12 h post-treatment was significantly induced.At other time points,its expression was similar to that of the control.Under SA treatment,GjCAD3 was significantly inhibited,and GjCAD5 expression was only significantly higher than that in the control at 24 h post-treatment.Under salt stress,GjCAD3 was significantly inhibited,whereas GjCAD5 was significantly induced.Both GjCAD3 and GjCAD5 were widely involved in gerbera abiotic stress responses,but their functions varied significantly.
作者
郭烨
李绅崇
王星淇
樊玥
武欢
卢珍红
杨春梅
程春振
GUO Ye;LI Shenchong;WANG Xingqi;FAN Yue;WU Huan;LU Zhenhong;YANG Chunmei;CHENG Chunzhen(Horticultural Biotechnology,College of Horticulture Institute,Fujian Agriculture and Forestry University,Fuzhou 350002,China;Flower Research Institute,Yunnan Academy of Agricultural Sciences,Kunming 650205,China;Yuxi Yunxing Biotechnology Co.,Ltd.,Yuxi 652604,China)
出处
《应用与环境生物学报》
CAS
CSCD
北大核心
2021年第5期1390-1398,共9页
Chinese Journal of Applied and Environmental Biology
基金
福建省高原学科建设经费项目(102/71201801101)
福建农林大学“校杰出青年科研人才”计划项目(xjq201721)资助。
关键词
非洲菊
肉桂醇脱氢酶
基因克隆
非生物胁迫
基因表达
gerbera
cinnamyl alcohol dehydrogenase
gene cloning
abiotic stress
gene expression
