摘要
从林下参叶片中分离获得45株内生细菌,采用对峙培养法筛选出1株对腐皮镰刀菌具有拮抗作用的菌株FS17,基于形态特征、生理生化特性和16S r RNA基因序列分析,鉴定该菌株为枯草芽孢杆菌(Bacillus subtilis)。采用生长速率法测定菌株FS17的发酵液对10种植物病原真菌均有抑制作用,抑菌率在46.83%~93.25%之间。菌株FS17发酵液处理人参根后,根腐病的发病率和病斑直径均明显小于对照,与多菌灵的防效差异不显著。本研究表明菌株FS17具有广谱抑菌性,可用于人参根腐病的生物防治。
From healthy mountain-cultivated ginseng leaves,45 endophytic bacteria strains were isolated.Antagonistic bacterium strain FS17 was screened with the confront culture method,and identified as Bacillus subtilis based on morphological characteristic and 16 S r RNA sequence analysis.Growth rate method was used to determine that the fermentation broth of FS17 had inhibitory effect on 10 plant pathogenic fungi,and the inhibition rate ranged from 46.83% to 93.25%.The fermentation broth of strain FS17 with lower disease incidence and lesion diameter had better control effects with no significant difference compared with that of carbendazim.This study suggests that strain FS17 showed strong inhibition effect and wide antagonistic spectrum,and could be useful to effectively control Fusarium solani causing ginseng root rot.
作者
周春元
宋娟
闫梅霞
郑培和
Zhou Chunyuan;Song Juan;Yan Meixia;Zheng Peihe(Institute of Special Animal and Plant Sciences of CAAS,Changchun,130112)
出处
《分子植物育种》
CAS
北大核心
2021年第20期6861-6866,共6页
Molecular Plant Breeding
基金
中国农业科学院科技创新工程项目(No.CAAS-ASTIP-2020-ISAPS)
国家重点研发计划项目(2017YFC17-02102)共同资助。
关键词
人参根腐病
拮抗细菌
鉴定
防效
Ginseng root rot
Antagonistic bacterium
Identification
Control effect
