摘要
铁是水稻必需的微量营养元素之一,但是土壤中积累过量亚铁盐会对水稻造成毒害作用。本研究通过生物信息学方法挖掘水稻Affymetrix表达谱芯片的数据,研究水稻种质Epagri108在铁毒胁迫处理和对照条件下的基因差异表达情况。结果显示,筛选到差异倍数在2倍及以上的基因407个,以对照处理组作为参照,铁毒胁迫条件下上调基因330个,下调基因77个。Gene Ontology和信号通路分析结果表明,这些差异表达基因主要涉及氧化还原反应、糖代谢、氨基酸代谢等生物学过程。本研究通过挖掘这些数据,初步探讨水稻在铁毒胁迫处理条件下的基因表达模式,为进一步揭示水稻耐受铁毒胁迫相关的分子机制提供了科学依据。
Iron is one of the essential micronutrients to rice,but the accumulation of excessive ferrous salt in soil can cause toxicity.In this study,bioinformatics method was used to mine the data of Affymetrix rice gene expression microarray to study the differentially expressed genes of rice germplasm Epagri108 under control and excess Fe conditions.The results revealed that 407 genes with more than two fold difference were identified.Compared to the control group,330 genes were up regulated and 77 genes were down regulated under excess Fe stress.Gene Ontology and pathway analysis revealed that these differentially expressed genes were mainly involved in the biological processes such as oxidoreductase activity,glucose metabolism,amino acid metabolism,etc.Through these data analysis,we preliminarily explored the gene expression patterns of rice under excess Fe conditions,and provided a scientific basis for further investgating the molecular mechanism tolerating for rice tolerance to Fe toxicity.
作者
刘慰华
寇姝燕
吴志刚
崔永祯
朱振华
袁平荣
Liu Weihua;Kou Shuyan;Wu Zhigang;Cui Yongzhen;Zhu Zhenhua;Yuan Pingrong(Food Crop Research Institute,Yunnan Academy of Agricultural Sciences,Kunming,650205)
出处
《分子植物育种》
CAS
北大核心
2021年第20期6624-6628,共5页
Molecular Plant Breeding
基金
云南省技术创新人才培养计划项目(2015HB107)
云南省云岭产业技术领军人才培养项目共同资助。
关键词
铁毒胁迫
基因芯片
差异表达基因
Fe toxicity
Gene chip
Differentially expressed genes
