摘要
背景:聚羟基丁酸戊酸共聚酯具有良好的生物相容性及降解性,但具有强疏水性,不利于细胞的黏附、生长、迁移和分化,严重限制了其在骨组织工程领域的应用,因此有必要对其进一步改性。目的:构建聚羟基丁酸戊酸共聚酯/半水硫酸钙/壳聚糖[poly(3-hydroxybutyrate-3-hydroxyvalerate)/calcium sulfate hemihydrate/chitosan,PHBV/CSH/CS]支架,探究其表征、模拟载药释放行为、生物安全性、体外成骨性和抗菌活性。方法:通过熔融沉积技术制备聚羟基丁酸戊酸共聚酯支架与聚羟基丁酸戊酸共聚酯/半水硫酸钙支架(半水硫酸钙为聚羟基丁酸戊酸共聚酯用量的20%),随后将聚羟基丁酸戊酸共聚酯/半水硫酸钙支架泡在壳聚糖溶液中并烘干,烘干后的支架放入超纯水中形成壳聚糖水凝胶并复合在支架表面上,制备PHBV/CSH/CS支架。观察3种支架的表面形貌、细胞黏附、细胞增殖情况;测试PHBV/CSH/CS支架的溶胀行为和模拟载药释放行为;利用茜素红染色评估3种支架的成骨性能;利用涂布平板法观察各组支架对标准金黄色葡萄球菌、标准大肠杆菌、临床金黄色葡萄球菌、临床大肠杆菌的抗菌能力。结果与结论:①所制备支架具有均匀且互连的多孔立体结构,平均孔径约为400μm,孔隙率为60%;②PHBV/CSH/CS支架溶胀率达56%,当使用牛血清白蛋白作为模型药物时,PHBV/CSH/CS支架载药率在1 h时达57.9%,6 h累计释放达56.5%;③聚羟基丁酸戊酸共聚酯支架不利于细胞黏附,聚羟基丁酸戊酸共聚酯/半水硫酸钙支架改善了细胞黏附,PHBV/CSH/CS支架显著提升了细胞黏附;④3种支架均可促进骨髓间充质干细胞增殖,3组均无明显细胞毒性;⑤茜素红染色实验表明,PHBV/CSH/CS支架对于骨髓间充质干细胞矿化结节的形成作用较另外两支架强;⑥体外抗菌实验表明,PHBV/CSH/CS支架对标准金黄色葡萄球菌、标准大肠杆菌、临床金黄色葡萄球菌、�
BACKGROUND:Poly(3-hydroxybutyrate-co-3-hydroxyvalerate)(PHBV)was a new type of polymer with good biocompatibility and degradability.However,PHBV has strong hydrophobicity,and was not conducive to cell adhesion,growth,migration,and differentiation,which severely limits its application in the field of bone tissue engineering.Thus,it is necessary to further modify it.OBJECTIVE:To construct PHBV/calcium sulfate hemihydrate(CSH)/chitosan(CS)scaffolds,and explore its characterization and drug release behavior,biological safety,osteogenic properties and antibacterial activity in vitro.METHODS:PHBV and PHBV/CSH scaffolds with 20 wt%CSH content were prepared by fused deposition technology.The PHBV/CSH scaffolds were immersed in the chitosan solution and dried,and the dried scaffolds were put into ultrapure water to form a chitosan hydrogel on the surface of the scaffolds,termed the PHBV/CSH/CS scaffolds.The surface morphology,cell adhesion,and cell proliferation of PHBV,PHBV/CSH,PHBV/CSH/CS scaffolds were observed.The swelling behavior and simulated drug release behavior of the PHBV/CSH/CS scaffolds were tested.Alizarin red staining was used to evaluate the osteogenic performance of these scaffolds.The spread plate method was used to observe the antibacterial ability of each group of scaffolds against standard Staphylococcus aureus,standard Escherichia coli,clinical Staphylococcus aureus,and clinical Escherichia coli.RESULTS AND CONCLUSION:(1)The scaffolds all had a uniform and interconnected porous three-dimensional structure,with an average pore size of about 400μm and a porosity of 60%.(2)The swelling rate of PHBV/CSH/CS scaffold was 56%.When using bovine serum albumin as a model drug,the loading rate of PHBV/CSH/CS scaffold was 57.9%at 1 hour,and the cumulative release at 6 hours was 56.5%.(3)PHBV scaffolds were not conducive to cell adhesion.PHBV/CSH scaffolds improved cell adhesion.PHBV/CSH/CS scaffolds significantly improved cell adhesion.(4)PHBV,PHBV/CSH,PHBV/CSH/CS scaffolds could promote the proliferation of bo
作者
叶翔凌
夏远军
王波群
康正阳
吴斌
Ye Xiangling;Xia Yuanjun;Wang Boqun;Kang Zhengyang;Wu Bin(Fifth Clinical College of Guangzhou University of Chinese Medicine,Guangzhou 510405,Guangdong Province,China;Department of Trauma Orthopedics,General Hospital of Southern Theater Command of PLA,Guangzhou 510010,Guangdong Province,China;School of Mechanical and Electrical Engineering,Dongguan Polytechnic,Dongguan 523808,Guangdong Province,China;Department of Orthopedics,Second People’s Hospital of Guangzhou Panyu,Guangzhou 510160,Guangdong Province,China)
出处
《中国组织工程研究》
CAS
北大核心
2022年第10期1574-1581,共8页
Chinese Journal of Tissue Engineering Research
基金
广州市科技计划项目(201804010136),项目负责人:夏远军。
关键词
3D打印
聚羟基丁酸戊酸共聚酯
半水硫酸钙
壳聚糖
水凝胶
生物相容性
抗菌活性
3D printing
poly(3-hydroxybutyrate-co-3-hydroxyvalerate)
calcium sulfate hemihydrate
chitosan
hydrogel
biocompatibility
antibacterial activity
