摘要
目的研究18β-甘草次酸钠(18β-SGA)对变应性鼻炎(AR)幼鼠及其对Th1/Th2失衡的改善作用及机制。方法50只SD幼鼠按随机数字表法分为对照组、模型组、18β-SGA低、高剂量组和地塞米松组,每组10只。除对照组外,其余大鼠根据卵清蛋白致敏法建立AR模型。造模后进行对应给药处理,持续4周。大鼠鼻部症状根据行为学评分法进行记录;ELISA法检测白细胞介素-2(IL-2)、干扰素-γ(IFN-γ)、免疫球蛋白E(IgE)以及IL-4、IL-5水平;HE染色观察大鼠鼻黏膜组织病理变化;免疫组化法检测鼻黏膜组织细胞间黏附分子-1(ICAM-1)、水通道蛋白5(AQP5)、NF-κB蛋白表达;Western blot检测鼻黏膜组织中NF-κB和PI3K-Akt信号通路相关蛋白表达。结果模型组大鼠较对照组大鼠评分显著降低(P=0.002),鼻粘膜组织出现明显血管扩张、水肿和严重炎性细胞浸润等病理现象。18β-SGA可减轻大鼠AR鼻部症状,抑制血清中IL-2、IFN-γ含量的下降及IgE、IL-4和IL-5含量的升高(P=0.001;P=0.003;P=0.008;P=0.002;P=0.002),下调鼻黏膜组织中ICAM-1、AQP5与NF-κB蛋白表达(P=0.002;P=0.004;P=0.005),并抑制p-PI3K、p-AKT及NF-κB p65的蛋白表达(P=0.006;P=0.002;P=0.002)。结论18β-SGA能减轻大鼠AR症状,其机制可能与抑制NF-κB和PI3K-Akt信号通路活性、调节体内Th1/Th2平衡相关。
Objective To study the effect and mechanism of 18β-glycyrrhetinic acid sodium(18β-SGA)on allergic rhinitis(AR)young mice and its improvement on Th1/Th2 imbalance.Methods Fifty young SD rats were randomly divided into control group,model group and 18 groupsβSGA low and high dose groups and dexamethasone group,with 10 rats in each group.Except the control group,the other rats established AR model according to ovalbumin sensitization method.The corresponding administration treatment was performed after modeling for 4 weeks.The nasal symptoms of rats were recorded according to the behavioral scoring method.Interleukin-2(IL-2)and interferon were detected by ELISA-γ(IFN-γ)、The levels of immunoglobulin E(IGE),IL-4 and IL-5.The histopathological changes of rat nasal mucosa were observed by HE staining.The intercellular adhesion molecule 1(ICAM-1),aquaporin 5(AQP5)and NF were detected by immunohistochemistryκWestern blot was used to detect NF in nasal mucosaκB and PI3K Akt signaling pathway related proteins were expressed.Results The score of the model group was significantly lower than that of the control group(P=0.002),and there were obvious pathological phenomena such as vasodilation,edema and severe inflammatory cell infiltration in the nasal mucosa.18βSGA can alleviate the nasal symptoms of AR in rats,inhibit IL-2 andγ-IFN in serum,increase the contents of IgE,IL-4 and IL-5(P=0.002,P=0.002),down regulate ICAM-1,AQP5 and NF in nasal mucosaκB protein expression(P=0.002;P=0.004;P=0.005),Inhibite P-PI3K,P-Akt and NFκprotein expression of B p65(P=0.006;P=0.002;P=0.002).Conclusion 18β-SGA can alleviate AR nasal symptoms in rats,and its mechanism may be related to inhibiting the activity of NF-κB and PI3K-Akt signaling pathway and regulating Th1/Th2 balance in vivo.
作者
符玉水
符元证
杨辉
钟丽花
霍开明
FU Yushui;FU Yuanzheng;YANG Hui;ZHONG Lihua;HUO Kaiming(Department of Neonatal, Hainan Women and Children's Medical Center, Haikou 571924, China;Department of Hygienic Toxicology, School of Public Health, China Medical University, Shenyang 110013, China;Department of Pediatrics, The Second Affiliated Hospital of Hainan Medical College, Haikou 570311, China)
出处
《西部医学》
2021年第11期1596-1601,1607,共7页
Medical Journal of West China
基金
海南省卫生健康行业科研项目(19A20078)。
