摘要
以‘白姜花’为母本,‘金姜花’为父本杂交获得的114株F1杂交后代为材料,对唇瓣主色、唇瓣心部主色、花丝主色和花药主色等4个重要花色性状进行观测和分析,同时利用集团分离分析法开发花色相关分子标记。结果显示,114株F1杂交后代花色分离广泛,唇瓣主色、唇瓣心部主色、花丝主色和花药主色的香农—维纳指数为0.57~1.58。唇瓣主色和唇瓣心部主色的L*、a*、b*值呈连续分布。聚类分析可将114个F1杂交后代分为橙色系和白色系两大类。BSA-SSR分析从191对引物中确定了HcgSSR676标记与花色性状紧密相关,该标记与白色系/橙色系,唇瓣主色黄度值(b*1)、唇瓣心部主色亮度值(L*2)、红度值(a*2)、黄度值(b*2)极显著相关,回归决定系数R2范围为7.92~23.19。
In this study,114 F_(1) hybrids derived from the cross between Hedychium coronarium‘COR01’and H.‘Jin’were used as materials.Four important flower color-related traits including the dominant color of labellum,labellum heart,filament,and anther were observed and analysed.Meanwhile,bulked segregant analysis(BSA)was used to develop color-related molecular markers.The results showed that the flower color of 114 F_(1) hybrids was widely separated,and the Shannon-Wiener index of the main color of labellum petal,labellum heart,filament,and anther ranged from 0.57 to 1.58.Colorimeter analysis indicated that the L^(*),a^(*) and b^(*) values of the dominant color of labellum and labellum heart were continuously distributed.Cluster analysis revealed that 114 F1 hybrids could be divided into white group and orange group.BSA-SSR analysis confirmed that the HcgSSR676 marker screened out from 191 primers pairs was closely related to flower color traits.The marker was significantly associated with the white/orange group,yellowness value of labellum dominant color(b*1),brightness value(L*2),redness value(a*2),and yellowness value(b*2)of labellum heart dominant color,whose regression determination coefficient(R2)ranged from 7.92 to 23.19.
作者
周熠玮
许国宇
王琴
严福龙
玉云祎
余让才
范燕萍
ZHOU Yiwei;XU Guoyu;WANG Qin;YAN Fulong;YU Yunyi;YU Rangcai;FAN Yanping(College of Forestry and Landscape Architecture,South China Agricultural University,Guangzhou 510642,China;Flower Research Center Guangzhou 510642,China;College of Life Science,South China Agricultural University,Guangzhou 510642,China)
出处
《园艺学报》
CAS
CSCD
北大核心
2021年第10期1921-1933,共13页
Acta Horticulturae Sinica
基金
广东省重点领域研发计划项目(2020B020220007)
国家自然科学基金项目(31770738)
广州市民生科技重点攻关计划项目(201903010054)。
关键词
姜花
花色
遗传分析
SSR
BSA
Hedychium
flower color
genetic analysis
SSR
BSA
