摘要
Recently,biomaterials for cartilage regeneration has been intensively investigated.However,the development of scaffolds that capture regenerated cartilage with biomechanical and structural recovery has rarely been reported.To address this challenge,platelet-rich plasma(PRP)-based cartilage constructs with a well-orchestrated symphony of cellular,biochemical and biomechanical elements were prepared by simultaneously employing chondrogenic progenitor cells(CPCs)as a cell source,optimizing platelet concentration,and adding an enzyme-ion activator.It was shown that this triple-optimized PRP+CPC construct possessed increased biomechanical properties and suitable biochemical signals.The following in vitro study demonstrated that the triple-optimized PRP+CPC constructs generated cartilage-like tissue with higher expression levels of chondrogenic-specific markers,more deposition of cartilage-specific extracellular matrix(ECM),and greater biomechanical values than those of the other constructs.Twelve weeks after the construct was implanted in a cartilage defect in vivo,histological analysis,qPCR,and biomechanical tests collectively showed that the triple-optimized constructs yielded a more chondrocyte-like cell phenotype with a higher synthesis of Col-Ⅱand aggrecan.More importantly,the triple-optimized constructs facilitated cartilage regeneration with better biomechanical recovery than that of the other constructs.These results demonstrate the efficacy of the triple-optimization strategy and highlight the simplicity and potency of this PRP+CPC construct for cartilage regeneration.
基金
the National Natural Science Foundation of China(81871771,81572159)
the Beijing Natural Sciences Grants(7182123,7191010).
