摘要
筛选红色诺卡氏菌(Nocardia rubra)Nr-8206株适宜投产的最佳培养形态。将红色诺卡氏菌Nr-8206株复壮,通过涂布、形态学考察筛选典型菌落形态。通过发酵技术获得各种菌落形态菌株的生物量,进一步通过细胞破碎、化学提纯等方法获得细胞壁多糖产物,紫外可见光分光光度法进行有效物质含量测定及杂质的检测。结果表明,红色诺卡氏菌Nr-8206株的最佳菌落形态为菌落直径1.68 mm、橘色、有突起、有褶皱、菌落边缘丝状,编号RY2。进行菌株RY2发酵,其菌体量最多,经破碎、提纯后,其有效物质糖含量及胞壁酸含量均高于其他形态的菌落,并高于出发菌株Nr-8206,且其杂质蛋白质残余量更低,杂质更容易去除。该研究可供生产企业以该菌株作为工作菌株时提供形态选择参考。
The best culturable form of Nocardia rubra Nr-8206 for production was screened.Strain Nr-8206 was rejuvenated,and the typical colony morphology was screened adopted spreading and morphology observation.Biomass of various form colonies were harvested through fermentation technology,and cell wall polysaccharide was further obtained by cell staving,chemical purification,and other methods,finally the content of effective materials and impurities were detected by UV-visible spectrophotometry.The results showed that the best colony morphology of N.rubra Nr-8206 was 1.68 mm in diameter,orange with protrusions and rimples,and the edges of colonies assumed filamentous,numbered as RY2.RY2 was selected for fermentation,and the results showed the bacterial cells of strain RY2 was the most among all the varies forms of colonies,and the content of effective material and teichoic acid were both higher than other colonies,and also higher than the starting strain Nr-8206 after cell staving and purification,at the same time the impurity protein residue was lower,and the impurities were easier to remove.This study could provide reference for the morphological selection for production enterprises when using this strain as a working strain.
作者
王丹
闫泉香
章朦玥
薛金艳
窦恒
王琳
张怡轩
WANG Dan;YAN Quan-xiang;ZHANG Meng-yue;XUE Jin-yan;DOU Heng;WANG Lin;ZHANG Yi-xuan(Schl.of Life Sci.&Biopharm.,Shenyang Pharm.Uni.,Shenyang 110016;Liaoning Greatest Bio-Pharm.Co.,Ltd,Benxi 117004;Shenyang Radio&TV Uni.,Shenyang 110003)
出处
《微生物学杂志》
CAS
CSCD
2021年第5期30-36,共7页
Journal of Microbiology
基金
辽宁省兴辽英才计划项目(XLYC1902072)
辽宁省重点研发计划指导计划项目(2017107013)。
关键词
放线菌
红色诺卡氏菌
筛选
含量测定
培养形态
杂质检测
Actinomycetes
Nocardia rubra
screening
bio-assay determination
culture form
impurity detection
