摘要
利用生物信息学方法筛选鉴定出28个FtERDs糖转运基因,其中19个编码蛋白序列且具有跨膜结构域.聚类分析将FtERDs归为4类,类Ⅰ包括13个,类Ⅱ包括9个,类Ⅲ包括1个,类Ⅳ包括5个.转录组测序检测到22个FtERDs在茎中表达,17个为差异表达基因(DEGs),其中仅3个DEGs在苗期10和15 d同时高表达,其余有3个DEGs在5 d高表达,2个DEGs在10 d高表达,9个DEGs在15 d高表达.这些基因可能在不同发育阶段的茎中发挥功能.同时,FtERDs间存在多个显著正相关或负相关,推测FtERDs基因的功能可能存在协同或互补效应.PEG模拟干旱胁迫8 h时,5个FtERDs过量表达,其中FtPinG34458.01、FtPinG85532.01表达量高于对照10倍以上.
A total of 28 early responsive to dehydration genes(ERDs) which regulated sugar transport in Fagopyrum tararicum were identified by bioinformatics method, and 19 FtERDs proteins were found to contain transmembrane domains. Clustering analysis showed that the FtERD genes can be classified into 4 groups, including 13 of type Ⅰ, 9 of type Ⅱ, 1 of type Ⅲ, and 5 of type Ⅳ. A total of 22 FtERDs, including 17 differentially expressed genes(DEGs), were detected in the stem of the seedlings based on transcriptome sequencing. There were only 3 DEGs which were both highly expressed on day 10 and 15 at the seedling stage. Other than that, another 3, 2 and 9 DEGs were highly expressed on day 5, 10 and 15 at the same period, respectively, implying the specific functions of FtERDs at different developmental stages in Tartary buckwheat. Furthermore, correlation analysis showed that there were several positively or negatively correlated FtERDs, suggesting the synergistic or complementary functions of FtERD genes. Five FtERDs were overexpressed 8 h after undergoing drought stress using PEG, among them the expression levels of FtPinG34458.01 and FtPinG85532.01 were over 10 times of the control.
作者
梁成刚
韦春玉
汪燕
关志秀
邓娇
黄娟
石桃雄
LIANG Chenggang;WEI Chunyu;WANG Yan;GUAN Zhixiu;DENG Jiao;HUANG Juan;SHI Taoxiong(Buckwheat Technology Research Center,School of Life Sciences,Guizhou Normal University,Guiyang,Guizhou 550001,China)
出处
《福建农林大学学报（自然科学版）》
CSCD
北大核心
2021年第6期734-740,共7页
Journal of Fujian Agriculture and Forestry University:Natural Science Edition
基金
国家自然科学基金项目(32060511、31660366)
贵州省科技支撑项目(黔科合ZC[2019]2298)
贵州师范大学博士启动基金项目
贵州省荞麦种质资源保育及创新重点实验室建设基金(黔教合KY[2017]002)。
关键词
苦荞
早期脱水响应基因
糖转运载体
生物信息学
基因表达
Tartary buckwheat
early responsive to dehydration
sugar transporter
bioinformatics
gene expression
