摘要
本试验旨在研究日粮中添加复合维生素B对生长期山羊消化道微生物组成和肠黏膜结构的影响。试验选取10只4月龄波杂雌山羊(12.60±1.28)kg,随机分为对照组(control,CON)和复合维生素B处理组(vitamin B,VB),CON组饲喂基础日粮(n=5),VB组在基础日粮中添加复合维生素B(n=5),预饲期2周,试验期11周,试验期间自由饮水。试验结束后屠宰采样,16S rRNA测序分析盲肠和结肠内容物微生物组成,检测肠道上皮组织形态结构和相关基因与蛋白的表达。通过菌群分类学分析发现,与CON相比,VB组山羊盲肠和结肠内容物菌群的α多样性指数无显著变化(P>0.05);在门水平上,盲肠和结肠内容物菌群中厚壁菌门(Firmicutes)和拟杆菌门(Bacteroidetes)为优势菌门,VB组盲肠拟杆菌门丰度有升高趋势(0.05<P<0.1),而螺旋菌门(Spirochaetes)丰度有降低趋势(0.05<P<0.1);在目水平上,VB组盲肠Solirubrobacterales和Streptomycetales目菌群丰度显著下降(P<0.05);在科水平上,VB组盲肠Christensenellaceae、Streptomycetaceae、Solirubrobacteraceae及结肠Christensenellaceae科菌群丰度显著下降(P<0.05);在属水平上,VB组盲肠Lachnoclostridium属菌群丰度显著升高(P<0.05),盲肠Bacillus、Nocardioides及结肠unidentified_(C)hristensenellaceae和unidentified_(G)ammaproteobacteria属菌群丰度显著下降(P<0.05)。与CON相比,VB组山羊空肠绒毛高度(P<0.01)、隐窝深度(P<0.05)和绒毛高度/隐窝深度(V/C,P<0.05)均显著增加;回肠隐窝深度显著降低(P<0.05),绒毛高度和V/C无显著变化(P>0.05)。实时荧光定量PCR结果显示,与CON相比,VB组山羊盲肠上皮紧密连接和细胞增殖相关基因的表达均无显著变化(P>0.05);而结肠黏膜上皮Occludin(P<0.05)、EGFR(P<0.01)、MIK67(P<0.05)和CCND(P<0.05)基因表达均显著上调;且VB组ZO-1蛋白表达显著上调(P<0.05)。综上表明,日粮中添加复合维生素B对生长期山羊肠道菌群多样性无显著影响,但可提高肠道中有益�
The aim of this study was to explore the effects of vitamin B complex on intestinal microflora composition and intestinal morphology and structure in growing goats. Ten four-month-old female goats with similar body weight(12. 60±1. 28 kg)were randomly divided into a control group(CON)and a group treated with vitamin B complex(VB). Goats in the CON group were fed a basal diet(n=5),and goats in the VB group were fed with the basal diet supplemented with vitamin B complex(n=5). The experiment comprised an adaptive feeding period of two weeks and a treatment period of 11 weeks. Throughout the experiment,all goats were provided free access to water. At the end of the experiment,the microflora composition of cecal and colonic contents was analyzed by 16 S rRNA sequencing,and the intestinal epithelial morphology structure and expression levels of related genes and proteins expression were detected. Compared to CON,VB supplementation did not alter the α diversity index obtained by bacterial taxonomic analysis(P>0. 05). At the phylum level,Firmicutes and Bacteroidetes were the dominant bacteria in the intestines of the goats. Compared to CON,there was a tendency for the abundance of Bacteroidetes in the cecum of the VB group to increase(0. 05<P<0. 10)and for the abundance of Spirochaetes to decrease(0. 05<P<0. 10). At the taxonomic order level,the abundance of Solirubrobacterales and Streptomycetales in the cecum of goats in the VB group was significantly decreased(P<0. 05). At the taxonomic family level,the abundance of Christensenellaceae,Streptomycetaceae,Solirubrobacteraceae in the cecum and Christensenellaceae in the colon of goats in the VB group was significantly decreased(P<0. 05). At the genus level, the abundance of Lachnoclostridium in the cecum of goats in the VB group was significantly increased(P<0. 05),while the abundance of Bacillus,Nocardioides in the cecum and unidentified_(C)hristensenellaceae and unidentified_(G)ammaproteobacteria in the colon of goats in the VB group was significantly decreased(P<0.
作者
吴晨
姚志浩
梅文晴
冯宇妍
陈渠
倪迎冬
WU Chen;YAO Zhi-hao;MEI Wen-qing;FENG Yu-yan;CHEN Qu;NI Ying-dong(Key Laboratory of Animal Physiology and Biochemistry,Ministry of Agriculture and Rural Affairs,College of Veterinary Medicine,Nanjing Agricultural University,Nanjing 210095,China)
出处
《草业学报》
CSCD
北大核心
2021年第11期170-180,共11页
Acta Prataculturae Sinica
基金
国家重点研发计划(2016YFD0501203-4)资助。
