摘要
目的研究固本化瘀法对2型糖尿病大鼠足细胞损伤及磷脂酰肌醇3-激酶/蛋白激酶B(PI3K/Akt)信号通路的影响。方法选择成年雄性SD大鼠并随机分为对照组,模型组,固本化瘀方低、中和高剂量组,采用高脂高糖饲料喂养联合链脲佐菌素腹腔注射的方式建立2型糖尿病模型,固本化瘀方低、中、高剂量组分别给予22.1、44.2、88.4 g·kg^(-1)固本化瘀方灌胃,连续4周,检测24 h尿微量白蛋白(mAlb)/尿肌酐(Ucr)、空腹血糖(FBG)、血肌酐(Scr)和血尿素氮(BUN)含量及肾脏病理改变、肾病蛋白(nephrin)、足细胞蛋白(podocin)、CD2相关蛋白(CD2AP)、磷酸化磷脂酰肌醇3-激酶(p-PI3K)和磷酸化蛋白激酶B(p-AKT)的表达水平。结果模型组大鼠的FBG、Scr、BUN、尿mAlb/Ucr均高于对照组,肾脏组织中nephrin、podocin、CD2AP、p-PI3K、p-Akt的表达水平均低于对照组;固本化瘀方低、中、高剂量组的FBG、Scr、BUN、尿mAlb/Ucr均低于模型组,肾脏组织中nephrin、podocin、CD2AP、p-PI3K和p-Akt的表达水平均高于模型组。结论固本化瘀法可减轻2型糖尿病大鼠足细胞损伤,该作用与激活PI3K/Akt通路有关。
Objective To study the effect of consolidating the root and resolving blood stasis method on podocyte injury and phosphatidylinositol 3-kinase and protein kinase B(PI3K/Akt)signaling pathway in type 2 diabetic rats.Methods Adult male SD rats were randomly divided into control group,model group,consolidating the root and resolving blood stasis prescription low-dose group,medium dose group and high-dose group.The model of type 2 diabetic was established by feeding high-fat and high sugar diet combined with intraperitoneal injection of streptozotocin.Consolidating the root and resolving blood stasis prescription was given by gavage at 22.1,44.2 and 88.4 g·kg^(-1) for 4 weeks.The fasting blood glucose(FBG),serum creatinine(Scr),blood urea nitrogen(BUN)and renal pathological changes,nephrin,podocin,CD2AP,p-PI3K,p-Akt expression levels were detected.Results FBG,Scr,BUN and urine mAlb/UCR of model group were higher than those of control group,and the expression levels of nephrin,podocin,CD2AP,p-PI3K and p-Akt in kidney tissue were lower than those of control group;FBG,Scr,BUN and urine mAlb/Ucr of low-dose group,middle dose group and high-dose group were lower than those of model group,and the expression levels of nephrin,podocin,CD2AP,p-PI3K and p-Akt in kidney tissue were higher than those of model group.Conclusion Consolidating the root and resolving blood stasis method can alleviate podocyte injury in type 2 diabetic rats,which relates to the activation of PI3K/Akt pathway.
作者
陆军
李红
LU Jun;LI Hong(Department of Pharmacy,Shanxi Hospital of Integrated Traditional and Western Medicine,Taiyuan 030013,China;The Second Department of Nephropathy,Shanxi Hospital of Integrated Traditional and Western Medicine,Taiyuan 030013,China)
出处
《西北药学杂志》
CAS
2021年第5期759-762,共4页
Northwest Pharmaceutical Journal
基金
山西中医药大学科技创新能力培育计划项目计划中医学术流派传承工作室建设项目(编号:2020PY-LP-10)。
关键词
2型糖尿病
糖尿病肾病
足细胞损伤
固本化瘀法
磷脂酰肌醇3-激酶/蛋白激酶B(PI3K/Akt)信号通路
type 2 diabetic
diabetic nephropathy
podocyte injury
consolidating the root and resolving blood stasis method
phosphatidylinositol 3-kinase and protein kinase B(PI3K/Akt)signaling pathway
