摘要
目的研究比较不同浓度干酵母、脂多糖注射诱导兔发热模型的体温变化特点,为基础实验提供参考。方法将30只2月龄新西兰雄兔随机分为5组,分别为正常组、20%干酵母组、10%干酵母组、0.5μg/m L脂多糖组、0.2μg/m L脂多糖组,每组各6只。干酵母组于造模前及造模后每1 h监测肛温至26 h、脂多糖组于造模前及造模后每20 min监测肛温至造模后6 h,记录体温与其他症状、体征的变化特点,绘制平均体温曲线,计算各时间点体温变化,比较各组体温变化趋势。结果皮下注射干酵母悬浊液,注射后2 h内体温下降,低于基础体温。2~4 h体温开始上升,6~8 h达峰值,高温可持续至少20 h,且随注射浓度的增加,升温值增大,峰值出现的时间延后。耳缘静脉注射脂多糖,40 min开始发热,体温曲线为双相热或三相热,致热后60~80 min达体温曲线第一峰,发热可持续至致热后240~300 min,且升温值与脂多糖注射剂量无明显关系,随注射剂量增大发热时程增长。结论干酵母和脂多糖是诱导兔发热的可靠模型,干酵母模型为长时、高热发热模型,脂多糖模型发热时程较短。应根据实验需要选择合适的致热模型。
Objective To provide a reference for basic experiments by studying and comparing the characteristics of body temperature changes in rabbit fever models induced by injection of various concentrations of dry yeast and lipopolysaccharide.Methods Thirty 2-month-old male New Zealand rabbits were randomly divided into five groups:a normal group,two groups injected with 10%or 20%dry yeast suspension,and two groups injected with 0.2 or 0.5μg/mL lipopolysaccharide solution with six rabbits in each group.The anal temperature of dry yeast groups was monitored every hour for 26 h before and after modeling,and that of lipopolysaccharide groups was monitored every 20 min for 6 h before and after modeling.The changes in body temperature and other symptoms were recorded,an average body temperature curve was drawn,changes in body temperature at each time point were calculated,and the trends of the body temperature change in each group were compared.Results Two hours after the dry yeast suspension was injected subcutaneously,body temperature decreased to lower than the basal body temperature.However,body temperature began to rise in 2~4 h,reached its peak in 6~8 h,then leveled off for at least 20 h.With the increase in concentration,the temperature rise was increased and the peak occurred later.Lipopolysaccharide was injected intravenously at the ear margin and fever began in40 min.The body temperature curve showed biphasic or triphasic heat and reached the first peak in 60~80 min and then leveled off for 240~300 min.The temperature rise had no obvious relationship with the injection dose of lipopolysaccharide,but the fever duration was increased with the increase in dose.Conclusions Dry yeast and lipopolysaccharide are reliable agents to induce fever in rabbits.The dry yeast model is a long-term high fever model,while the lipopolysaccharide model has a short fever time.The appropriate model to induce fever should be selected in accordance with the experimental requirements.
作者
张英琦
焦谊
于天源
刘志凤
刘迪
王厚融
徐亚静
官乾
ZHANG Yingqi;JIAO Yi;YU Tianyuan;LIU Zhifeng;LIU Di;WANG Hourong;XU Yajing;GUAN Qian(Beijing University of Chinese Medicine,Beijing 100029,China;Dongfang Hospital of Beijing University of Chinese Medicine,Beijing 100078)
出处
《中国比较医学杂志》
CAS
北大核心
2021年第10期16-21,共6页
Chinese Journal of Comparative Medicine
基金
国家自然科学基金项目(81873392)
北京市自然科学基金(7192113)。
关键词
发热
动物模型
兔
体温
fever
animal model
rabbit
body temperature
