摘要
目的探讨建立新西兰兔全膝关节置换术后假体周围感染模型的方法及可行性。方法选取健康新西兰兔48只,随机分成3组:实验组(假体周围感染组)、对照组(假体未感染组)及空白对照组(假手术组),每组各16只;实验组左膝关节全膝关节置换术后7 d于新西兰兔关节腔内注射0.5 m L金黄色葡萄球菌悬液(1×10^(7)CFU/m L),对照组左膝关节全膝关节置换术后7 d于新西兰兔关节腔内注射0.5 m L生理盐水,空白对照组左膝关节囊切开缝合术后未进行任何处理。各组分别于术前和术后7、21 d进行血液学检查,术后21 d进行X线检查、细菌学及病理学检查。结果术后21 d实验组成活率87.5%(14/16),对照组及空白对照组成活率100%。术后21 d X线检查显示实验组及对照组均显示关节假体在位,未见假体周围松动,实验组关节周围软组织肿胀,对照组及空白对照组关节周围软组织肿胀不明显;血液学检查结果显示实验组白细胞总数(WBC)、ESR和CRP较对照组及空白对照组高,有统计学差异(P<0.05);细菌学检查显示实验组细菌培养结果均为阳性,感染率100%(14/14),对照组细菌培养阳性1例,感染率6.3%(1/16);空白对照组细菌培养均为阴性,感染率0%(0/16);病理学检查显示实验组假体周围及周围组织有大量炎性细胞浸润;对照组及空白对照组假体周围及周围组织无炎性细胞浸润。结论新西兰兔行全膝关节置换术后7 d,关节腔内注射0.5 m L金黄色葡萄球菌悬液,可成功建立人工关节置换术后感染模型。
Objective To explore the method and feasibility of establishing a peri-prosthetic infection model after total knee arthroplasty in New Zealand rabbits.Methods Forty-eight healthy New Zealand rabbits were randomly divided into three groups:an experimental group(peri-prosthetic infection group),a control group(non-peri-prosthetic infection group),and a blank control group(sham surgery group).A bacterial suspension of Staphylococcus aureus(0.5 m L,1×10^(7) CFU/m L)was injected into the joint cavity of New Zealand rabbits 7 d after total knee arthroplasty in the experimental group,while 0.5 m L saline was injected into the joint cavity of New Zealand rabbits 7 d after the total knee arthroplasty in the control group,and no treatment was undertaken after the capsule incision and suture of the knee joint in the blank control group.Hematology examination was performed 1 day before surgery and 7,14,and 21 d after surgery in each group.X-ray examination,bacteriology,and pathology examination were performed 21 d after surgery.Results The survival rate of the experimental group was 87.5%(14/16),and that of the control group and the blank control group was 100%.Twenty-one days after surgery,X-ray examination showed that the prosthesis was good in the experimental group and the control group,and there was no loosening around the prosthesis.The soft tissue around the joint was swollen in the experimental group,but not in the control group and the blank control group.Hematology examination showed that WBC,ESR,and CRP were significantly higher in the experimental group than in the control and blank control groups(P<0.05).The bacteriological examination showed that the bacterial culture was positive in the experimental group,and the infection rate was 100%(14/14),while the infection rate was 6.3%(1/16)in one case in the control group,and the bacterial culture was negative in the blank control group,with an infection rate of 0%(0/16).Pathological examination showed that there was a large amount of inflammatory cell infiltration a
作者
王勇平
张怀斌
谢瑞敏
陶贵彦
侯卫华
刘小荣
WANG Yongping;ZHANG Huaibin;XIE Ruimin;TAO Guiyan;HOU Weihua;LIU Xiaorong(Department of Orthopaedics,the First Hospital of Lanzhou University,Lanzhou 730000;China.2.Department of Laboratory,College of Clinical Medicine of Northwest University for Nationalities,Lanzhou 730000)
出处
《中国实验动物学报》
CAS
CSCD
北大核心
2021年第5期657-663,共7页
Acta Laboratorium Animalis Scientia Sinica
基金
甘肃省卫生行业科研计划项目(GSWSHY-2015-53)
中央高校基本科研业务费项目(31920200021)。
关键词
关节置换
假体周围感染
细菌
新西兰兔
joint replacement
peri-prosthetic infection
bacteria
New Zealand rabbit
