摘要
目的观察豚鼠脑缺血再灌注损伤(IRI)后螺旋神经节细胞形态、结构的改变和脑源性神经营养因子(BDNF)表达的变化,探讨BDNF对脑IRI后螺旋神经节细胞的营养保护作用。方法将48只健康豚鼠随机分成正常组、假手术组和IRI 6 h、12 h、24 h、48 h组,用灼断两侧椎动脉、夹闭一侧颈总动脉的方法建立豚鼠IRI模型。光镜下观察螺旋神经节的病理改变,透射电镜观察螺旋神经节细胞超微结构的改变;免疫组织化学法和Western blot检测BDNF表达部位和表达量的变化。结果光镜和透射电镜观察表明脑IRI导致螺旋神经节细胞数量减少和细胞损伤,IRI 24 h细胞的损伤最为严重;Western blot检测显示各组豚鼠螺旋神经节细胞都有BDNF表达,正常组、假手术组分别为0.390±0.021和0.381±0.019,两组表达差异无统计学意义(P>0.05),IRI 6 h组(0.423±0.041)表达增加,差异有统计学意义(P<0.05),随后各组表达量减少,IRI 24 h组最少(0.218±0.081),差异有统计学意义(P<0.05);免疫组织化学法与Western blot检测结果一致,并显示BDNF表达部位在细胞浆。结论脑IRI可导致耳蜗螺旋神经节细胞损伤和BDNF表达量的改变,BDNF可能参与了IRI引发的螺旋神经节细胞损伤的病理过程。
Objective To explore the changes of the structure of spiral ganglion cells and the expression of brainderived neurotrophic factor(BDNF)after cerebral ischemia-reperfusion injury(IRI)in guinea pigs,and to explore the nutritional protective effect of BDNF on normal and post-IRI spiral ganglion cells.Methods A total of 48 healthy guinea pigs were randomly divided into six groups:normal group,sham operation group,ischemia-reperfusion injury 6 h(IRI 6 h)group,ischemia-reperfusion injury 12 h(IRI 12 h)group,ischemia-reperfusion injury 24 h(IRI 24 h)group and ischemia-reperfusion injury 48 h(IRI 48 h)group.The pathomorphological changes were analyzed with hematoxylin-eosin staining and ultrastructural changes of spiral ganglion cells observed by transmission electron microscopy.Meanwhile,the expression of BDNF was observed and determined by immunohistochemisty and Western blot analysis.Results Light microscopy and transmission electron microscopy showed that cerebral IRI in guinea pigs resulted in the decrease of the number of spiral ganglion cells and cell damage,and the most serious injury was found in spiral ganglion cells at IRI 24 h group.BDNF was expressed in the cytoplasm of spiral ganglion cells in all groups,and the expression level of BDNF in sham-operated group(0.381±0.019)was the same as that in normal group(0.390±0.021)(P>0.05).The expression increased in 6 h group(0.423±0.041),then decreased,and the lowest in 24 h group(0.218±0.081),the difference was statistically significant(P<0.05).The results of immunohistochemistry were consistent with those of Western blot analysis.Conclusion Cerebral ischemia-reperfusion injury in guinea pigs can lead to the damage of spiral ganglion cells,and BDNF may be involved in the pathological process of cerebral IRI-induced damage of spiral ganglion cells.
作者
许险艳
林迳苍
李德水
丁小明
XU Xian-yan;LIN Jing-cang;LI De-shui;DING Xiao-ming(Quanzhou Medical College,Quanzhou,Fujian Province 362000,China)
出处
《解剖学研究》
CAS
2021年第5期492-496,共5页
Anatomy Research
基金
泉州医学高等专科学校校级重点科研项目(XJ1621)
泉州市科技计划项目(2014Z74)。
关键词
耳蜗
螺旋神经节细胞
缺血再灌注损伤
脑源性神经营养因子
豚鼠
Cochlea
Spiral ganglion cell
Ischemia-reperfusion injury
Brain derived neurotrophic factor
Guinea pigs
