摘要
目的探索维生素C(VC)对PM2.5诱导的肺脏炎性损伤的缓解作用及其分子机制。方法32只雄性SD大鼠随机分为:空白对照(control group,CON)、阴性对照(negative group,NEG)、PM2.5染毒(PM 2.5 exposure group,PM2.5)和VC干预(vitamin C group,VC)共4组。CON组无任何处理,NEG组采用生理盐水气管滴注和灌胃,PM2.5组采用PM2.5悬浊液气管滴注同时生理盐水灌胃,VC组采用PM2.5悬浊液气管滴注同时VC灌胃。动物饲养与干预共21d,每日进行灌胃,气管滴注于D8、D10、D12进行。大鼠处死后采集肺脏和血清,HE染色及酶学试剂盒评估肺脏组织炎性损伤和氧化应激状态,同时使用Western blot和ELISA法检测NLRP3炎症小体信号通路中关键蛋白的表达水平。结果肺组织HE染色表明CON组与NEG组未出现明显的炎性损伤,而PM2.5组肺脏炎性损伤显著,VC组炎性液体渗出和炎细胞浸润较PM2.5组明显减轻。同时,与PM2.5组相比,VC组肺脏中ROS和MDA含量降低而SOD、GSH-Px活力增强。此外,VC组肺脏中TLR4、NLRP3、ASC以及Caspase-1四种炎症关键蛋白水平较PM2.5组降低,并且其下游产物IL-1β和IL-18在血清中的水平同样降低。结论,VC可以缓解因PM2.5造成的肺部炎性损伤,其机制可能与降低ROS水平、抑制NLRP3炎症小体通路有关。
Objective To explore the protective effect of vitamin C(VC)against lung inflammation induced by PM2.5 exposure in rats.Methods Thirty-two SD rats were randomly divided into 4 groups:control group(CON),negative group(NEG),PM2.5 exposure group(PM2.5),and VC group.CON group did not receive any treatment.NEG group received saline via tracheal instillation and gavage.PM2.5 group received PM2.5 suspension via tracheal instillation and saline via gavage.VC group received PM2.5 suspension via tracheal instillation and VC via gavage.The experiment lasted 21 days,and the gavage operation was conducted every day.Besides,the tracheal instillation was conducted at days 8,10 and 12.Finally,Rats were euthanized,and the lung and serum samples were collected.The status of the inflammatory injury and oxidative stress was evaluated using HE staining and test kits.The expressions of key proteins involved in the NLRP3 inflammasome signaling pathway were examined using Western blot and ELISA.Results There is no inflammatory injury in rats in CON and NEG groups,while rats in PM2.5 group exhibited significant lung inflammation.The treatment of VC alleviated fluid the leakage of fluid and inflammatory infiltration in the lung.Moreover,VC group shows a decrease in ROS and MDA levels and an increase in SOD and GSH-Px activities in the lung.Furthermore,the expressions of TLR4,NLRP3,Caspase-1 and ASC in lung,as well as the levels of downstream products(IL-1βand IL-18)in serum were significantly lowered in VC group than in PM2.5 group.Conclusion VC could alleviate lung inflammation induced by PM2.5 exposure,and the underlying mechanism is related to decreased ROS level and inhibited NLRP3 inflammasome signaling pathway.
作者
李航宇
张寒凝
梁璐瑶
吕帅帅
韩长城
马玉霞
LI Hang-yu;ZHANG Han-ning;LIANG Lu-yao;LV Shuai-shuai;HAN Chang-cheng;MA Yu-xia(Department of Nutrition and Food Hygiene,School of Public Health.Hebei Medical University,Hebei Province Key Laboratory of Environment and Population Health,Shijiazhuang 050017,China)
出处
《营养学报》
CAS
CSCD
北大核心
2021年第4期389-394,共6页
Acta Nutrimenta Sinica
基金
国家自然基金资助项目(No.81874264)。
