摘要
【目的】通过对水稻剑叶性状的综合评价,明确剑叶相关性状间及与6个农艺性状的关系。检测剑叶相关性状的QTL,为优良株型品种选育,剑叶性状基因的精细定位和克隆奠定基础。【方法】以日本优质粳稻品种越光和葡萄牙粳稻地方种Bertone构建的回交群体两个世代为实验材料,利用BC3F1群体基因型构建遗传连锁图谱;测定亲本和BC3F2群体各株系剑叶SPAD、剑叶长、剑叶宽,计算剑叶长宽比、剑叶面积;利用隶属函数和标准差系数赋予权重法获得剑叶性状综合评价值(D值),分析其与6个农艺性状间的关系。分别利用单标记分析(SPA)和区间作图(IM)检测水稻剑叶相关性状QTL。【结果】在抽穗灌浆期,两亲本剑叶SPAD值呈现先升高后降低的动态变化。BC3F2群体的5个剑叶相关性状变异丰富,总体表现趋向轮回亲本越光。4个剑叶形态性状间相关性均达到极显著水平,与剑叶SPAD的相关性不显著。主成分和逐步线性回归分析表明剑叶宽、剑叶SPAD、剑叶长、剑叶面积是影响剑叶综合评价值(D值)的主要因子。高D值株系的株高、穗长、茎基粗和单株产量均极显著高于低D值株系,两者的分蘖数和有效穗数差异不显著。共检测到18个控制剑叶性状的QTL,分布在水稻第1、4、7和8染色体上,贡献率分布范围为4.00%~28.00%(SPA)和3.41%~27.00%(IM),除qFLSPAD1之外的17个QTL增效基因均来自Bertone。在第8染色体上的RM22720-RM404区间发现1个QTL簇,含6个主效QTL,分别为q FLL8.1、q FLL8.2、qFLA8.1、q FLA8.2、qD8.1和q D8.2。【结论】获得了剑叶宽、剑叶SPAD、剑叶长和剑叶面积4个评价剑叶性状的关键指标;明确了剑叶性状与单株产量之间的正相关关系;检测到18个剑叶相关性状QTL,位于第8染色体RM22720-RM404区间的QTL簇,是影响剑叶性状的1个重要染色体区域。
【Objective】In order to clarify the correlations between flag leaf-related traits and their relationships with six agronomic traits, a comprehensive evaluation of flag leaf traits was made. The identification of QTLs associated with flag leaf traits will lay a foundation for fine mapping and cloning of the functional genes underlying rice flag leaf-related traits,which is helpful to breed elite cultivars with excellent flag leaf traits. 【Method】A backcross population with two generations derived from an elite japonica rice cultivar Koshihikari(recipient) and a Portuguese rice landrace Bertone(donor) was used as plant materials. The genetic linkage maps were constructed by using SSR markers with the BC3 F1 population. Several parameters including flag leaf SPAD, flag leaf length, flag leaf width, length to width ratio of flag leaf as well as flag leaf area were measured in the parental varieties and BC3 F2 population. In order to analyze the relationship between D value and the six agronomic traits, membership functions and the index weight method were used to comprehensively evaluate the flag leaf traits of these 260 BC3 F2 lines. Single-point analysis(SPA) and interval mapping(IM) were used to identify QTLs for traits related to flag leaf in rice.【Result】After the flag leaf was fully expanded, the flag leaf SPAD values of the two parents showed a dynamic change, increasing at first and reduced subsequently. The five flag leaf-related traits of the BC3 F2 population exhibited diverse phenotypic variations, whose overall performance resembled the recurrent parent(Koshihikari). The results revealed that four morphological traits of flag leaf were significantly correlated with each other, while there were no significant correlations with flag leaf SPAD.Using principal component analysis(PCA) and stepwise regression analysis, flag leaf width, flag leaf SPAD, flag leaf length and flag leaf area were selected as the key indexes of D value out of the flag leaf comprehensive evaluation. The results showed that
作者
李杰
田蓉蓉
白天亮
朱春艳
宋佳伟
田蕾
马帅国
吕建东
胡慧
王震宇
罗成科
张银霞
李培富
LI Jie;TIAN Rongrong;BAI Tianliang;ZHU Chunyan;SONG Jiawei;TIAN Lei;MA Shuaiguo;LÜJiandong;HU Hui;WANG Zhenyu;LUO Chengke;ZHANG Yinxia;LI Peifu(College of Agriculture,Ningxia University/Key Laboratory of Modern Molecular Breeding for Dominant and Specific Crops in Ningxia,Yinchuan 750021,China;Baiyin Municipal Bureau of Agriculture and Rural Affairs,Baiyin 730900,China)
出处
《中国水稻科学》
CAS
CSCD
北大核心
2021年第6期573-585,共13页
Chinese Journal of Rice Science
基金
国家自然科学基金资助项目(31760374)
宁夏自然科学基金资助项目(2020AAC03096)
宁夏农业育种专项(2018NYYZ0301,2018NYYZ0302)。
关键词
水稻
回交群体
剑叶性状
综合评价
QTL定位
rice
backcross population
flag leaf traits
comprehensive evaluation
QTL mapping
