摘要
[目的]建立超高效液相色谱-串联质谱法(UPLC-MS/MS)定量检测动物源性食品中16种非甾体消炎类兽药残留量的方法。[方法]样品用甲酸-水-乙腈混合溶液提取,净化后经C 18色谱柱分离,UPLC-MS/MS法测定。[结果]样品由基质匹配标准曲线进行定量,所检测的16种非甾体消炎药在1~200 ng/g线性关系良好(R^(2)>0.995)。分别添加1倍定量限、2倍定量限和10倍定量限3个水平的混合标准溶液进行回收率试验,各个浓度水平加标回收率在70%~105%,相对标准偏差(RSD)均在15%以下(n=6)。[结论]该方法简便、稳定、准确,具有良好的重现性,适用于测定动物源性食品中16种非甾体消炎类兽药残留量。
[Objective]To establish a method for the quantitative determination of 16 non-steroidal anti-inflammatory drugs(NSAIDs)in animal-origin foods by ultra performance liquid chromatography-tandem mass spectrometry(UPLC-MS/MS).[Method]The samples were extracted with a mixture of formic acid-water-acetonitrile.After purification,the samples were separated by C 18 column and quantitatively detected by UPLC-MS/MS.[Result]The samples were quantified by matrix matching standard curves.The 16 NSAIDs had good linear relationships in the range of 10-200 ng/mL(R^(2)>0.995).The mixed standard solutions of 1,2 and 10 times of the limits of quantification(LOQ)were added respectively for the recovery experiments,the recovery rates of each concentration level were 70%-105%,and the relative standard deviation(RSD)were all less than 15%(n=6).[Conclusion]The method is simple,stable,accurate and reproducible.It is suitable for the determination of 16 NSAIDs in animal-origin foods.
作者
方志娟
李晓芹
丁洪流
何新叶
金萍
王伟
FANG Zhi-juan;LI Xiao-qin;DING Hong-liu(Suzhou Institute for Food Control,Suzhou,Jiangsu 215104;Suzhou Institute of Product Quality Supervision and Inspection,Suzhou,Jiangsu 215104)
出处
《安徽农业科学》
CAS
2021年第21期211-214,217,共5页
Journal of Anhui Agricultural Sciences
基金
苏州市科技计划项目(SS2019039,SS202038)。
关键词
超高效液相色谱-串联质谱法
非甾体消炎类药
动物源性食品
兽药残留
Ultra performance liquid chromatography-tandem mass spectrometry(UPLC-MS/MS)
Non-steroidal anti-inflammatory drugs(NSAIDs)
Animal-origin foods
Veterinary drug residue
