摘要
建立了兽用林可霉素注射液中非法添加盐酸左旋咪唑的高效液相色谱检测方法。采用Waters AtlantisT3 C18(4.6×250 mm,5μm)色谱柱分离,以磷酸二氢钠溶液-甲醇(70∶30)作为流动相进行等度洗脱(流速1.00 mL/min),用二极管阵列检测器在230 nm波长处进行测定,考察此色谱条件下方法的专属性、线性、回收率、精密度、检测限和耐用性。结果表明,盐酸左旋咪唑在进样浓度为0.02~0.4 mg/mL时呈良好的线性关系,R2=0.9999;重复性试验RSD为0.6%;平均回收率为99.7%;检测限为0.0002 mg/mL,定量限为0.0016 mg/mL。该方法准确、简便、可行,适用于林可霉素注射液中盐酸左旋咪唑的检测。
A method was established to detect the levamisole hydrochloride adulterated into veterinary lincomycin hydrochloride injection by high performance liquid chromatography(HPLC).Isocratic elution was used with the mobile phase consisted of sodium dihydrogen phosphate buffered saline solution and methanol at the volume ratio of 70∶30,and its constant flow rate was 1.00 mL/min.It was performed on the Waters AtlantisT3(4.6×250 mm,5μm)C18 column at 230 nm by diode-array detector.Specificity,linear relation,recovery,precision,detection limit and durability were investigated under the chromatographic condition.The results showed that there was good linear relation between the levamisole hydrochloride concentrations and peak areas in the range of 0.02 mg/mL~0.4 mg/mL,R2=0.9999.The RSD of precision was 0.6%.The average recovery rate was 99.7%.Limit of detection was 0.0002 mg/mL,limit of quantatation is 0.0016 mg/mL.This method is simple,accurate,feasible,so it is suitable for detection of levamisole hydrochloride which is illegally adulterated into lincomycin injection.
作者
郑洁
刘雪红
陈玲
ZHENG Jie;LIU Xue-hong;CHEN Ling(Dalian Inspection and Certification Technology Service Center, Dalian,Liaoning 116021, China)
出处
《中国兽药杂志》
2021年第10期25-30,共6页
Chinese Journal of Veterinary Drug
