摘要
目的:探讨siRNA沉默黏蛋白(mucin,MUC)16基因对人胆囊癌细胞(GBC-SD)增殖和迁移的影响及其分子机制。方法:采用慢病毒感染和siRNA干扰技术沉默GBC-SD中MUC16基因(GBC-SD+MUC16-siRNA组),同时设立阴性对照组(GBC-SD+NC组)和空白对照组(GBC-SD组)。RT-PCR检测各组GBC-SD细胞中MUC16表达情况,噻唑蓝实验检测GBC-SD细胞增殖能力,划痕实验检测GBC-SD细胞迁移能力,蛋白免疫印迹实验检测GBC-SD细胞中PCNA和CD44蛋白表达情况。结果:GBC-SD+MUC16-siRNA组细胞增殖率和细胞迁移数目均低于GBC-SD组(P<0.05);GBC-SD+MUC16-siRNA组MUC16、CD44蛋白表达均低于GBC-SD组、GBC-SD+NC组(P<0.05~P<0.01),PCNA蛋白表达亦低于GBC-SD+NC组(P<0.05)。结论:siRNA沉默MUC16具有一定的抑制胆囊癌细胞增殖和迁移作用,可为胆囊癌的治疗提供新的实验证据。
Objective:To explore the effects of siRNA silencing mucin 16(MUC16)on the proliferation and migration of gallbladder carcinoma GBC-SD cells,and its molecular mechanisms.Methods:The GBC-SD+MUC16-siRNA group was constructed using the lentivirus infection and siRNA interference technique to silence MUC16 gene in GBC-SD cells,and the negative control group(GBC-SD+NC group)and blank control group(GBC-SD group)were set up simultaneously.The RT-PCR was used to detect the expression of MUC16 in GBC-SD cells,the MTT and scratch assays were used to detect the proliferation and migration ability of GBC-SD cells,and the Western blotting was used to detect the protein expressions of CD44 and PCNA in GBC-SD cells.Results:The cell proliferation rate and cell migration number in GBC-SD+MUC16-SiRNA group were lower than those in GBC-SD group(P<0.05).The expression levels of MUC16 and CD44 protein in GBC-SD+MUC16-SIRNA group were lower than those in GBC-SD group and GBC-SD+NC group(P<0.05 to P<0.01),and the expression level of PCNA protein in GBC-SD+MUC16-SIRNA group was also lower than that in GBC-SD+NC group(P<0.05).Conclusions:SiRNA silencing MUC16 can inhibit the proliferation and migration of gallbladder cancer cells,which may provide the new experimental evidence for the treatment of gallbladder cancer.
作者
夏泽亮
刘言玉
褚亮
刘言浩
王法宝
周超毅
蒋磊
XIA Ze-liang;LIU Yan-yu;CHU Liang;LIU Yan-hao;WANG Fa-bao;ZHOU Chao-yi;JIANG Lei(Department of General Surgery,The Second Affiliated Hospital of Bengbu Medical College,Bengbu Anhui 233004;ICU,The First Affiliated Hospital of Bengbu Medical College,Bengbu Anhui 233004,China;Institute for Clinical Trials of Drugs,The First Affiliated Hospital of Bengbu Medical College,Bengbu Anhui 233004,China)
出处
《蚌埠医学院学报》
CAS
2021年第10期1345-1348,共4页
Journal of Bengbu Medical College
基金
蚌埠医学院自然科学项目(BYKY2019138ZD,BYKY2019153ZD,BYKY2019136ZD)。
关键词
胆囊肿瘤
黏蛋白
细胞增殖
细胞迁移
gallbladder neoplasms
mucin
cell proliferation
cell migration
