摘要
基于拟南芥(Arabidopsis thaliana(L.)Heynh)单角果种子数量(角粒数)的全基因组关联分析(GWAS)数据,筛选到一个可能影响角粒数的候选基因AtMES1,并对其表达模式和转录组数据进行了分析。结果显示,AtMES1的表达模式分析结果表明其在心皮和花序处等特异性表达。分析该基因T-DNA插入突变体的表型发现其角粒数比野生型对照显著下降。转录组分析结果表明,参与调控胚珠发生和发育的多个重要基因在缺失突变体中表达下调。同时使用胎座特异启动子使AtMES1在雌蕊中过量表达,发现转基因植株的角果长度变短,但种子密度显著增加,原因可能是通过激活植物激素相关通路,从而调控胚珠发生和发育相关基因表达正调控角粒数。研究结果初步证实了AtMES1具有正调控拟南芥角粒数和种子密度的功能。
According to our previous genome-wide association analysis(GWAS)and prediction of gene expression,we identified AtMES1 as a potential gene that may participate in regulating seed number per silique(SNS).We identified the loss-of-function mutants of AtMES1 and analyzed their phenotypes.AtMES1 had significantly reduced SNS and seed density compared with the wild-type control.Transcriptomic analysis revealed that multiple important genes involved in hormone-related ovule initiation and development were down-regulated,suggesting that AtMES1 positively regulated SNS by modulating hormone signals.The ectopic expression of AtMES1 by placenta-specific promoter STK led to shorter siliques but significantly increased seed density,further demonstrating that AtMES1 positively regulated ovule initiation and seed density.In conclusion,our study suggests that AtMES1 is a new positive regulator of Arabidopsis ovule initiation and SNS.
作者
常金慧
潘健
姜欢利
林文慧
Chang Jin-Hui;Pan Jian;Jiang Huan-Li;Lin Wen-Hui(School of Life Sciences and Biotechnology,Shanghai Jiao Tong University, Shanghai 200240, China;School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai 200240, China)
出处
《植物科学学报》
CAS
CSCD
北大核心
2021年第5期506-514,共9页
Plant Science Journal
基金
国家自然科学基金项目(31771591,32070342)
上海交通大学农工交叉基金项目(Agri-X20200204)。
关键词
AtMES1基因
角粒数
种子密度
转录组测序
AtMES1
Seed number per silique(SNS)
Seed density
Transcriptome sequencing
