摘要
目的考察骨康宁洗剂的急性毒性和重复给药毒性。方法12只家兔随机分为空白对照组、完整皮肤组、破损皮肤组,单次贴敷最大剂量后观察急性毒性反应。重复给药毒性实验,80只豚鼠随机分为空白对照组和低、中、高剂量组,经皮重复贴敷给药30 d,停药14 d,给药期和停药期结束时观察一般状态及体质量,检测血液学指标、血液生化指标、脏器系数及组织病理学等指标。结果急性毒性实验中,家兔皮肤无红斑、水肿和渗出,未见异常毒性反应和死亡。重复给药毒性实验中,各组豚鼠体质量、血液学指标、血液生化指标、脏器系数、组织病理学检查未见明显的异常变化。结论在所设实验条件下,骨康宁洗剂未引起明显的毒性反应。
Objective To investigate the acute toxicity and repeated dose toxicity of Gukangning Lotion.Methods 12 rabbits were randomly divided into control group, intact skin group and damaged skin group.Acute toxicity were observed after administration.For repeated dose toxicity, 80 guinea pigs were randomly divided into control group and the low, middle, high dose group, and the guinea pig were administrated for 30 days and recovery for 14 days.The general situation, body weight, hematological indexes, biochemical indexes, organ coefficients and histopathological changes were observed and tested.Results For acute toxicity, the skin of rabbits was normal, no death was recorded and no obvious toxic events were observed.For the repeated dose toxicity, no obvious abnormalities was found in body weight, hematological indexes, biochemical indexes, organ coefficients and histopathological test.Conclusion Gukangning Lotion has no obvious toxicity under the present experimental conditions.
作者
张晓萌
王巨才
陈国彪
王康俊
ZHANG Xiaomeng;WANG Jucai;CHEN Guobiao;WANG Kangjun(Hainan Key Laboratory for Pharmaceutical Quality Research,Hainan Institute for Drug Control,Haikou 570216,China)
出处
《沈阳药科大学学报》
CAS
CSCD
北大核心
2021年第9期942-949,共8页
Journal of Shenyang Pharmaceutical University
基金
国家科技支撑计划项目(2007BAI27B00)。
关键词
骨康宁洗剂
急性毒性
重复给药毒性
Gukangning Lotion
acute toxicity
repeated dose toxicity
