摘要
目的建立QuEChERS结合高效液相色谱法(high performance liquid chromatography,HPLC)测定食品接触用耐高温纸制品中14种有机抗菌剂的方法。方法样品用乙腈-水溶液(1:1, V:V)超声提取, QuEChERS方法净化, C18色谱柱(250 mm×4.6 mm, 5μm)分离,以乙腈、甲醇、0.1%乙酸溶液为流动相进行梯度洗脱,二极管阵列检测器于191~400 nm检测,外标法定量。结果 14种有机抗菌剂在0.05~20.00 mg/L范围内呈良好线性关系,r≥0.996;方法检出限均为0.05mg/kg,定量限均为0.15mg/kg;平均加标回收率为73.8%~96.3%,相对标准偏差为2.8%~5.5%。结论该方法操作简单、快速、准确可靠,适用于食品接触用耐高温纸制品中14种有机抗菌剂的检测。
Objective To establish a method for the determination of 14 kinds of organic antimicrobials in high temperature resistant paper products for food contact by QuEChERS combined with high performance liquid chromatography(HPLC). Methods The samples were extracted ultrasonically with acetonitrile-water solution(1:1, V:V), purified by QuEChERS, separated by C18(250 mm×4.6 mm, 5 μm) column combined with acetonitrile, methanol and 0.1% acetic acid solution as mobile phases for gradient elution, detected by diode array detector(DAD) at 191-400 nm, and quantified by external standard method. Results The calibration curves of the 14 kinds of organic antimicrobials showed good linear relationships in the range of 0.05-20.00 mg/L with the correlation coefficients great than or equal to 0.996. The limits of detection(LODs) and the limits of quantification(LOQs) of the 14 kinds of organic antimicrobials were 0.05 mg/kg and 0.15 mg/kg, respectively. The average recoveries were in the range of 73.8%-96.3% with relative standard deviations(RSDs) in the range of 2.8%-5.5%. Conclusion The method is simple, fast, accurate and reliable, and suitable for the detection of the 14 kinds of organic antimicrobials in high temperature resistant paper products for food contact.
作者
伍尚森
綦艳
李锦清
黄翠莉
冉继伟
WU Shang-Sen;QI Yan;LI Jin-Qing;HUANG Cui-Li;RAN Ji-Wei(Guangdong Testing Institute of Product Quality Supervision,Shunde 528300,China)
出处
《食品安全质量检测学报》
CAS
北大核心
2021年第18期7229-7234,共6页
Journal of Food Safety and Quality
基金
广东省市场监督管理局科技项目(2018CZ07)。
关键词
QUECHERS
高效液相色谱法
食品接触用纸
耐高温纸制品
有机抗菌剂
QuEChERS
high performance liquid chromatography
food contact paper
high temperature resistant paper products
organic antimicrobials
