摘要
通过接种某城市污水处理厂好氧池生物膜,采用NH_(4)^(+)-N+NO_(2)^(-)-N(SMBBR-1)和NH_(4)^(+)-N+NO_(3)^(-)-N+HAc(SMBBR-2)两种进水基质启动厌氧氨氧化序批式移动床生物膜反应器(Sequencing Moving Bed Biofilm Reactor,SMBBR),研究不同基质条件下反应器的启动特性.结果表明,两反应器在运行100 d后均成功启动并稳定运行,在进水负荷分别为0.83和0.32 kg·m^(-3)·d^(-1)(以N计)的条件下,氮去除率分别达到81.82%±1.20%和66.35%±4.79%.活性测定结果显示,SMBBR-1和SMBBR-2中Anammox活性分别达到6448.32和1980.32 mg·m^(-2)·d^(-1),表明Anammox菌被成功富集.高通量结果显示,SMBBR-1和SMBBR-2中启动成功后的Anammox菌由Ca.Brocadia和Ca.Jettenia组成,其中,Ca.Brocadia占比分别为11.02%和7.57%,Ca.Jettenia占比分别为2.07%和0.56%.除Anammox菌外,SMBBR-2中还包括Thauera(2.84%)和Flavobacterium菌(0.66%),其为部分反硝化菌的主导菌属.本研究表明,虽然两种不同基质的启动办法各有利弊,但其均能实现厌氧氨氧化SMBBR的启动,可为主流系统内的Anammox菌快速富集培养提供技术支撑.
To investigate the characteristics of anammox enrichment with different substrates,two SMBBRs with aerobic biofilm taken from municipal wastewater treatment were established.One was fed with ammonium and nitrite(SMBBR-1),the other was fed with ammonium,nitrate and acetic acid(SMBBR-2).The long-term operation results showed 81.82%±1.20% and 66.35%±4.79%of nitrogen removal efficiencies were stably obtained under the nitrogen loading rate(NLR)of 0.83 and 0.32 kg·m^(-3)·d^(-1) for SMBBR-1 and SMBBR-2 after 100 days'operation.The anammox activities of SMBBR-1 and SMBBR-2 could reach 6448.32 and 1980.32 mg·m^(-2)·d^(-1),respectively,which suggested that anammox bacteria were successfully enriched in two SMBBRs.The high-throughput sequencing results revealed Ca.Brocadia and Ca.Jettenia were the predominance for anammox bacteria in SMBBR-1 and SMBBR-2.The relative abundance of Ca.Brocadia were 11.02% and 7.57%,and the abundance of Ca.Jettenia were 2.07% and 0.56%,respectively.The Thauera and Flavobacterium were also detected with relative abundance of 2.84% and 0.66% in SMBBR-2,which were the dominated species of partial denitrifying bacteria.This study showed that enrichment methods with different substrates for anammox SMBBR start-up had the advantages and disadvantages.However,these two substrates strategies could be used for reactor establishment and provided support for anammox enrichment in mainstream.
作者
姚雪薇
邵贤明
王康舟
吕恺
彭党聪
YAO Xuewei;SHAO Xianming;WANG Kangzhou;Lu Kai;PENG Dangcong(School of Municipal and Environmental Engineering,Xi'an University of Architecture and Technology,Xi'an 710055;Key Laboratory of Northwest Water Resource,Environment and Ecology,Ministry of Education,Xi'an 710055)
出处
《环境科学学报》
CAS
CSCD
北大核心
2021年第10期4049-4055,共7页
Acta Scientiae Circumstantiae
基金
陕西省重点研发计划(No.2019ZDLSF06-05)。
关键词
厌氧氨氧化
富集培养
移动床生物膜反应器(SMBBR)
部分反硝化
anaerobic ammonium oxidation(anammox)
enrichment
sequencing moving bed biofilm reactor(SMBBR)
partial denitrification
