摘要
本文在固定化葡葡糖氧化酶方面,对几种固定化载体和方法做了最佳条件摸索和比较。改进了戊二醛偶联法,尝试用二乙醇胺先保护戊二醛一端,另一端与载体偶联,然后脱去保护基再偶联酶,效果较好。用该法制得固定化酶在所研究的三种方法中效果最好。固定化酶活力回收率比用聚丙烯酰胺包埋法高出一倍多,比用重氮盐共价键合法高出10%左右。
In this study,the bcsi condition of immobilized glucose oxidase using different supports and different methods was studied,and compared.An improved method of glucose oxidase immobilization method using glutaric dialdehyde was studied.One end of the glutaric dialdehyde molecule was first protected by diethanol-amine and the other end was linked to the carrier.Then the protected end was hydrolysed and glucose oxidase was linked to it.Clucose oxidase prepared according to the above method had the highest activity.The enzyme activity was 10% more than the didzo-reaction method and twice nigher than the polyacrylamide entrapment method.
作者
李丽霞
鲁格
陈长治
俞耀庭
Li Lixia;Lu Ge;Chen Changzhi;Yu Yaoting(Institute of Moleclar Biology,Nankai University,Tianjin)
出处
《高等学校化学学报》
SCIE
EI
CAS
1987年第6期562-567,共6页
Chemical Journal of Chinese Universities
