摘要
四倍体刺槐因其具有适应性强、生物量大等特点,被广泛推广种植。本研究采用展开子叶的刺槐幼苗为材料,通过5种浓度的秋水仙素处理茎尖,诱导刺槐四倍体,并研究诱导后四倍体叶片解剖结构的变化。研究发现0.05%的秋水仙素处理幼苗1 d能取得较好的诱导效果。四倍体刺槐的叶片解剖结构中,上表皮厚度、栅栏组织、海绵组织和叶片厚度等都显著高于二倍体,四倍体叶片紧实度显著提高,表明刺槐四倍体化后有较强的抗逆性。研究结果可为刺槐多倍体育种提供可靠的技术支持,并为刺槐的倍性育种工作提供参考和理论支持,同时为阐述四倍体刺槐抗逆性形成原理提供形态解剖学依据。
Tetraploid Robinia pseudoacacia L. has been widely planted because of its strong adaptability and large biomass. In this study, the seedlings of R. pseudoacacia were treated with 5 different concentrations colchicine to induce tetraploid, and the changes of the anatomical structure of the tetraploid leaves after induction were studied. It was found that 0.05% colchicine could achieve better induction effect in one day. The thickness of the upper epidermis, the palisade tissue, sponge tissue and leaf thickness of the tetraploid R. pseudoacacia leaves were significantly higher than those of diploid, and the cell tense ratio of tetraploid ones were significantly improved, which indicated that R. pseudoacacia had strong resistance to stress after tetraploidization. The results can provide reliable technical support for the polyploid breeding of R. pseudoacacia, provide reference and theoretical support for the ploidy breeding of R. pseudoacacia, and provide morphological and anatomical basis for explaining the formation principle of the resistance of tetraploid R. pseudoacacia.
作者
张丞慧
孟冰超
杨茂磊
马洪峥
胡宝全
Zhang Chenghui;Meng Binchao;Yang Maolei;Ma Hongzheng;Hu Baoquan(College of Horticulture and Landscape,Tianjin Agricultural University,Tianjin 300392,China)
出处
《天津农学院学报》
CAS
2021年第3期18-21,共4页
Journal of Tianjin Agricultural University
基金
国家自然科学基金(3180011064)
天津市大学生创新训练计划项目(201810061251)。
关键词
刺槐
四倍体
秋水仙素
叶片
解剖结构
Robinia pseudoacacia L.
tetraploid
colchicine
leaves
anatomical structure
