摘要
氰丙氨酸合成酶(β-cyanoalanine synthase,β-CAS)是植物氰化物解毒的关键酶,在调节植物生长发育及逆境胁迫中扮演重要角色。本研究从巴西橡胶树中克隆氰丙氨酸合成酶基因HbCAS,并对其进行分析。结果表明:HbCAS基因开放阅读框长为1113 bp,编码370个氨基酸,其理论分子量为40.15 kDa,等电点为8.90,属于色氨酸合成酶超家族。实时荧光定量PCR分析结果显示,HbCAS基因在橡胶树各种组织中均有表达,其中在胶乳中的表达量最高。同健康树相比,HbCAS基因在死皮树中的表达显著下调。过氧化氢及乙烯利、茉莉酸甲酯、水杨酸及脱落酸等多种激素均能调控HbCAS基因的表达。同时,HbCAS基因的表达也受干旱、低温、甲基紫精、高盐等多种非生物胁迫调节。本研究结果揭示HbCAS基因可能在橡胶树死皮发生、活性氧信号、激素调节及多种非生物胁迫应答中起重要作用。
β-cyanoalanine synthase(β-CAS),a key enzyme involved in cyanide detoxification in plants,plays an important role in regulating the growth and development and stress response.In this study,HbCAS was cloned from H.brasiliensis.The results indicated that HbCAS had an open reading frame(ORF)of 1113 bp,encoding 370 amino acids with a theoretical molecular weight 40.15 kDa and an isoelectric point of 8.90,belonging to the superfamily of tryptophan synthase beta.Quantitative real-time PCR analysis showed HbCAS was found to express in all tested tissues with the highest expression in latex.Compared with the healthy trees,HbCAS expression significantly decreased in the tapping panel dryness(TPD)infected trees.Hydrogen peroxide(H2O2)and various hormones of ethephon(ETH),Methyl jasmonate(MeJA),salicylic acid(SA)and abscisic acid(ABA)all regulated the expression of HbCAS.Meanwhile,HbCAS expression was regulated by diverse abiotic stresses of drought,low temperature,methyl viologen and high salt.These results suggest that HbCAS might play key roles in TPD onset,reactive oxygen species signaling,hormone regulating,as well as various abiotic stresses responses.
作者
张议文
刘辉
冯成天
胡义钰
王真辉
袁坤
ZHANG Yiwen;LIU Hui;FENG Chengtian;HU Yiyu;WANG Zhenhui;YUAN Kun(College of Tropical Crop,Hainan University,Haikou,Hainan 570228,China;Rubber Research Institute,Chinese Academy of Tropical Agricultural Sciences/Key Laboratory of Biology and Genetic Resources of Rubber Tree,Ministry of Agriculture and Rural Affairs/State Key Laboratory Incubation Base for Cultivation&Physiology of Tropical Crops,Haikou,Hainan 571101,China)
出处
《热带作物学报》
CSCD
北大核心
2021年第9期2451-2457,共7页
Chinese Journal of Tropical Crops
基金
中央级公益性科研院所基本科研业务费专项(No.1630022020010)
国家天然橡胶产业技术体系(No.CARS-34-GW5)。
关键词
巴西橡胶树
氰丙氨酸合成酶
死皮
胁迫应答
Hevea brasiliensis
β-cyanoalanine synthase
tapping panel dryness
stress response
