摘要
南京某化工厂生产邻硝基对甲基苯酚和邻氨基对甲基苯酚,场地调查和室内微宇宙实验研究发现该场地发生了1,2,4-三氯苯(1,2,4-TCB)污染,且场地内天然发生着1,2,4-TCB的厌氧还原脱氯反应。为探究该场地地下水中邻硝基对甲基苯酚和邻氨基对甲基苯酚对1,2,4-TCB厌氧脱氯过程的影响,开展了3组微宇宙实验:(1)底物仅为1,2,4-TCB,(2)底物为1,2,4-TCB和邻氨基对甲基苯酚,(3)底物为1,2,4-TCB和邻硝基对甲基苯酚。对比了3种不同底物条件下1,2,4-TCB的降解过程、降解速率及微生物群落结构。实验结果表明:(1)邻硝基对甲基苯酚在厌氧条件下还原生成邻氨基对甲基苯酚,同时抑制1,2,4-三氯苯厌氧生物降解;最终1,2,4-TCB降解产物只有1,4-DCB。(2)邻氨基对甲基苯酚不会阻碍1,2,4-TCB的降解,但会降低降解速率。(3)Azospira和Pseudomonas与邻硝基对甲基苯酚还原反应有关,尤其是Pseudomonas极有可能是场地邻硝基对甲基苯酚还原的功能菌。
In a Nanjing chemical plant manufacturing o-nitro-p-methylphenol and o-amino-p-methylphenol,field investigation and microcosm study found contaminated groundwater where the contaminant,1,2,4-trichlorobenzene(1,2,4-TCB),was biodegraded by indigenous anaerobic microbes.In this study,we conducted three groups of microcosm experiments to explore the effects of o-nitro-p-methylphenol and oamino-p-methylphenol on the 1,2,4-TCB dechlorination process,using(1)1,2,4-TCB alone,(2)1,2,4-TCB and o-amino-p-methylphenol,or(3)1,2,4-TCB and o-nitro-p-methylphenol as the substrate(s),and compared the degradation processes,degradation rates,and microbial communities for the three groups of experiments.The results show:(1)O-nitro-p-methylphenol was reduced to o-amino-p-methylphenol while 1,2,4-TCB dechlorination was inhibited,with 1,4-DCB as the only dechlorination product.(2)O-amino-pmethylphenol did not prohibit 1,2,4-TCB degradation but decreased degradation rate.(3)Azospira and Pseudomonas were involved in o-nitro-p-methylphenol reduction,especially Pseudomonas was probably the functional microorganism under the anaerobic condition at the site.
作者
刘芷彤
周妮
乔文静
叶淑君
LIU Zhitong;ZHOU Ni;QIAO Wenjing;YE Shujun(Ministry of Education Key Laboratory of Surficial Geochemistry,School of Earth Sciences and Engineering,Nanjing University,Nanjing 210023,China;Jiangsu Academy of Environmental Industry and Technology Corp.,Nanjing 210000,China;College of Life Sciences,Nanjing Agricultural University,Nanjing 210032,China)
出处
《地学前缘》
EI
CAS
CSCD
北大核心
2021年第5期159-166,共8页
Earth Science Frontiers
基金
国家自然科学基金项目“地下水中氯代有机溶剂的厌氧生物研究(42077174)”。
