摘要
采用超声辅助双水相提取槟榔多糖,研究料液比、超声温度、超声时间对槟榔多糖提取率的影响,并采用响应面法优化提取工艺条件。结果表明,选用质量比2∶1的(NH4)2SO4-乙醇作为最佳的双水相体系,超声辅助双水相法提取槟榔多糖最佳工艺为料液比1∶20(g/mL)、超声温度70℃、超声时间40 min,槟榔多糖的提取率达到(4.52±0.25)%。DPPH·、ABTS^(+)·、·OH清除试验表明,在一定的浓度范围内,槟榔多糖具有较好的抗氧化性,且呈现良好的量效关系。
Areca catechu L.polysaccharides(ACPs)were extracted by ultrasonic-assisted aqueous two-phase extraction.Response surface methodology was used to optimize the liquid-material ratio,extraction temperature,and extraction time for maximal extraction yield.An(NH4)2SO4-ethanol mass ratio of 2∶1 was selected as the best aqueous two-phase system.The optimal conditions were found to be extraction at 70℃for 40 min with a solid-to-solvent ratio of 1∶20(g/mL).Experiments conducted under these conditions gave a polysaccharide yield of(4.52±0.25)%.DPPH·,ABTS^(+)·and·OH antioxidant assays indicated that ACPs had good antioxidant activity within a certain concentration range,and showed a good dose-effect relationship.
作者
尹明松
丁贺辉
潘飞兵
匡凤姣
冀晓龙
刘延奇
YIN Ming-song;DING He-hui;PAN Fei-bing;KUANG Feng-jiao;JI Xiao-long;LIU Yan-qi(College of Food and Bioengineering,Zhengzhou University of Light Industry,Zhengzhou 450001,Henan,China;Huachuang Institute of Areca Research-Hainan,Haikou 571600,Hainan,China;Hainan Kouweiwang Science and Technology Development Co.,Ltd.,Wanning 571542,Hainan,China)
出处
《食品研究与开发》
CAS
北大核心
2021年第19期163-170,共8页
Food Research and Development
基金
海南华创槟榔研究院院长基金(HCBL2020YZ-010)
郑州轻工业大学博士科研启动基金(2020BSJJ015)。
关键词
超声辅助
双水相提取
槟榔
多糖
抗氧化
ultrasonic-assisted
aqueous two-phase extraction
Areca catechu L.
polysaccharide
antioxidant
