摘要
目的探讨化学发光免疫分析法(CLIA)在酶联免疫吸附试验(ELISA)检测人类免疫缺陷病毒(HIV)抗体阳性的无偿献血者归队中应用的可行性。方法收集ELISA筛查HIV呈阳性反应性并自愿咨询检测的无偿献血者100例,进行CLIA检测的同时送当地疾病预防控制中心进行确证,并将两种方法的检测结果与疾病预防控制中心确证结果进行比较分析。结果CLIA检测结果阳性符合率显著高于ELISA(P<0.01)。当CLIA结果S/CO值≥100.0时,阳性符合率显著提高;而ELISA检测只有当两种试剂结果均为阳性且S/CO值≥10时,确证结果才为阳性。结论CLIA用于检测ELISA筛查HIV阳性的无偿献血者,对无偿献血者队伍的管理具有重要意义。
Objective To explore the feasibility of applying chemiluminescence in the return voluntary blood donors HIV-positive in ELISA test.Methods A total of 100 cases of voluntary blood donors who was HIV-positive in preliminary screening and voluntarily consulted the test were samples.The samples underwent CLIA test and were simultaneously sent to local center for disease prevention and control for confirmation.The test results of these two methods were compared with the confirmatory results of the center for disease prevention and control.Results The positive coincidence rate of chemiluminescence was significantly higher than that of ELISA,and the difference was statistically significant(P<0.01);The positive coincidence rate increased significantly when the S/CO value of CLIA was≥100.0.However,in ELISA test,only when the results of the agents were all positive and S/CO≥10,the confirmatory result is positive.Conclusion It is of great significance for the management of the team of voluntary blood donors to apply CLIA in testing voluntary blood donors HIV-positive in ELISA screening.
作者
于艳涛
李交
符发雄
叶伟亮
刘志泉
梁丽雯
朱慧娟
陈永飞
罗娇娜
欧阳海宁
YU Yan-tao;LI Jiao;FU Fa-xiong;YE Wei-liang;LIU Zhi-quan;LIANG Li-wen;ZHU Hui-juan;CHEN Yong-fei;LUO Jiao-na;OUYANG Hai-ning(Laboratory Department,Central Blood Center of Shunde,Foshan 528300,China)
出处
《广东医科大学学报》
2021年第5期587-589,共3页
Journal of Guangdong Medical University
基金
佛山市自筹经费类科技计划项目(No.1920001001675)。
关键词
化学发光法
HIV抗体
无偿献血者
chemiluminescence
HIV antibody
voluntary blood donors
