摘要
目的探讨新疆农牧区老年男性RANK基因甲基化与衰弱的关系。方法以2018年4~5月新疆农牧区65岁以上男性常住居民横断面流行病学调查为基础,从中随机抽取90例,其中哈萨克族30例,维吾尔族28例,汉族32例。通过问卷调查收集研究对象的基本信息,并采集空腹外周血5 mL,提取DNA。采用重亚硫酸盐测序法检测RANK基因启动子区甲基化率。采用Fried衰弱表型评估研究对象是否衰弱,将衰弱者作为病例组,衰弱前期及无衰弱者(非衰弱者)作为对照组。应用协方差分析分析RANK基因启动子区CpG岛甲基化率与衰弱的相关性。结果哈萨克族、汉族、维吾尔族对照组与病例组组内的RANK基因CpG岛甲基化率比较,差异均无统计学意义(P>0.05);3个民族的对照组RANK基因CpG岛甲基化率均明显高于病例组(P<0.05)。在校正BMI、饮酒、吸烟、年龄、高血压、糖尿病后,协方差分析结果依然显示新疆农牧区病例组的RANK基因CpG岛甲基化率显著低于对照组(P<0.05)。结论新疆农牧区老年男性的衰弱可能与RANK基因甲基化率降低相关。
Objective To investigate the correlation between RANK gene methylation and frailty in the elderly men in agricultural and pastoral areas of Xinjiang.Methods This study was based on the epidemiological cross-sectional investigation of male residents aged over 65 years old in agricultural and pastoral areas of Xinjiang from April to May 2018,and 90 cases were randomly selected,including 30 Kazak,28 Uygur and 32 Han.General information was obtained by questionnaire,and 5 mL of fasting peripheral blood were collected to extract DNA.The methylation rate of the promoter region of RANK gene was identified by bisulfite genomic sequence.Fried phenotye was used to evaluate whether the subjects were frailty or not.The subjects with frailty were taken as the case group and the others without frailty as the control group.The correlation between the methylation rate of CpG island in the promoter region of RANK gene and frailty was analyzed by covariance analysis.Results There was no difference in the methylation rate of RANK gene among the control groups and case groups of Kazak,Uygur or Han(P>0.05).The methylation rates of RANK gene in the case groups of the three nationalities were significantly lower than those in the control groups of the three nationalities(P<0.05).After adjusting body mass index,drinking,smoking,age,hypertension and diabetes,the covariance analysis still showed that the methylation rate of RANK gene in the case group was significantly lower than that in the control group(P<0.05).Conclusions Among the elderly men in agricultural and pastoral areas of Xinjiang,the decreased methylation rate of RANK gene may be associated with frailty.
作者
卓娅·买买提乌斯满
向红
白雪
李杨静
王红梅
ZHUO-YA Mai-mai-ti-wu-si-man;XIANG Hong;BAI Xue;LI Yang-jing;WANG Hong-mei(Second Department of Cadre Health Care Center, Xinjiang Uygur Autonomous Region People’s Hospital, Urumqi 830001, China)
出处
《实用老年医学》
CAS
2021年第10期1016-1018,1022,共4页
Practical Geriatrics
基金
国家自然科学基金资助项目(81660242)。
