摘要
目的:基于网络药理学探究人参-甘松药对治疗阵发性房颤有效成分的靶基因及作用机制。方法:通过TCMSP中药数据库检索人参-甘松药对的化学成分,以生物利用度(OB)≥30%和类药性(DL)≥0.18为条件筛选潜在有效成分,并通过TCMSP数据库预测潜在靶点。运用Uniprot数据库找到潜在靶点相对应的人类基因名称,通过Genecards数据库检索阵发性房颤的疾病靶点,与药对的潜在靶点相映射取得交集靶点,绘制韦恩图,通过Cytoscape3.7.2构建疾病-药物-成分-靶点网络图。利用STRING数据库构建PPI蛋白互作网络图选出核心靶标,最后进行GO分析和KEGG分析。结果:共获得28个有效成分和45个有效靶点。GO富集分析发现,主要途径有神经递质受体活性、离子门控通道活性、被动跨膜转运蛋白活性、G蛋白偶联神经递质受体活性等。KEGG通路富集分析发现,主要通路为IL-17信号通路、钙信号通路、TNF信号通路等。结论:人参-甘松药对通过多靶点、多途径、多种信号通路协同发挥治疗阵发性房颤的作用,为进一步研究药物作用机制提供基础,并为指导临床提供依据。
Objective:To explore the target gene and mechanism of effective components of Renshen-Gansong in the treat-ment of paroxysmal atrial fibrillation based on network pharmacology.Methods:The chemical components of Renshen-Gansong drug pairs were searched by TCMSP traditional Chinese medicine database.The potentially effective components were screened under the conditions of bioavailability(OB)≥30%and drug-like(DL)≥0.18,and the potential targets were predicted by TC-MSP database.The human gene name corresponding to the potential target was found by Uniprot database,and the disease target of paroxysmal atrial fibrillation was searched by Genecards database.The intersection target was mapped with the potential target of drug pair,the Wayne diagram was drawn,and the disease-drug-component-target network map was constructed by Cytoscape3.7.2.The PPI protein interaction network map was constructed by STRING database to select the core targets,and finally GO analysis and KEGG analysis were carried out.Results:A total of 28 active components and 45 effective targets were obtained.GO enrichment analysis showed that the main pathways were neurotransmitter receptor activity,ion gated channel activity,passive transmembrane transporter activity,G protein coupled neurotransmitter receptor activity,et al.KEGG pathway enrichment analysis showed that the main pathways were IL-17 signal pathway,calcium signal pathway,TNF signal pathway and so on.Conclusion:Renshen-Gansong has a synergistic effect on the treatment of paroxysmal atrial fibrillation through multi-targets,multi-pathways and multiple signal pathways,which provides a basis for further study of the drug mechanism and clinical guidance.
作者
郭经奇
李莹
范吉林
任雯庆
薛振宇
张世亮
GUO Jing-qi;LI Ying;FAN Ji-lin;REN Wen-qing;XUE Zhen-yu;ZHANG Shi-liang(Shandong University of Traditional Chinese Medicine,Jinan 250014,China;Affiliated Hospital of Shandong University of Traditional Chinese Medicine,Jinan 250014,China)
出处
《海南医学院学报》
CAS
2021年第20期1574-1580,共7页
Journal of Hainan Medical University
基金
国家科技重大专项重大新药创制项目(2017ZX09301003)。
关键词
网络药理学
人参
甘松
阵发性房颤
作用机制
Network pharmacology
Renshen
Gansong
Paroxysmal atrial fibrillation
Mechanism of action
