摘要
2019年9月上旬,吉林延边地区某养羊场羊群陆续出现以精神萎靡、食量减少、流鼻涕、咳嗽、腹泻为主要症状的发病情况。为了确定引起该养羊场发病羊感染的病原菌及筛选有效的治疗药物,试验采集患病羊的鼻拭子样本,进行细菌分离,并对分离菌进行革兰氏染色、形态学观察、生化鉴定、PCR鉴定、16S rDNA测序后进行同源性比对和遗传进化分析,对分离菌株进行药敏试验。结果表明:从患病羊鼻拭子样本中分离得到1株在LB固体培养基上呈金黄色、隆起、光滑、湿润、边缘整齐的圆形菌落,革兰氏染色阳性,其生化培养特性与金黄色葡萄球菌符合,16S rDNA核苷酸序列经BLAST比对后与金黄色葡萄球菌相似性为100%。分离菌株对头孢喹肟、卡那霉素、庆大霉素、强力霉素、四环素、氯霉素、红霉素、万古霉素敏感,对螺旋霉素、青霉素、氨苄西林、洁霉素耐药。指导该养羊场使用头孢喹肟后,病羊痊愈。说明通过药敏试验选取敏感药物防治羊金黄色葡萄球菌病非常必要。
In early September 2019,the main symptoms of malaise,reduced appetite,runny nose,cough,and diarrhea appeared in the flock of a sheep farm in Yanbian area,Jilin Province.In order to determine the pathogenic bacteria affecting sheep in the sheep farm and to screen effective treatment drugs,the test was carried out on the nasal swab samples of the diseased sheep,and the bacteria were separated;and Gram staining,morphological observation,biochemical identification,PCR identification,16S rDNA sequencing were performed on the isolated bacteria.Homology comparison and genetic evolution analysis were carried out,and drug sensitivity test was used for the isolated bacteria.The results showed that one strain was isolated from the nasal swab samples of the diseased sheep,which was golden yellow,raised,smooth,moist,neat edge,and round colonies on the LB solid medium and Gram-positive.The bacteria were consistent with the biochemical culture characteristics of Staphylococcus aureus.BLAST alignment with 16S rDNA nucleotide sequence showed 100%similarity with Staphylococcus aureus.The isolated strain was sensitive to cefquinome,kanamycin,gentamicin,doxycycline,tetracycline,chloramphenicol,erythromycin,vancomycin,and resistant to spiramycin,penicillin,ampicillin,lincomycin.After instructing the sheep farm to use cefquinome,the sick sheep recovered.The results suggested that it was very necessary to select sensitive drugs to prevent and treat sheep Staphylococcus aureus through drug susceptibility test.
作者
张燕
郑添予
金春梅
周博宇
梁晚枫
于龙政
ZHANG Yan;ZHENG Tianyu;JIN Chunmei;ZHOU Boyu;LIANG Wanfeng;YU Longzheng(Agricultural College,Yanbian University,Yanji 133002,China)
出处
《黑龙江畜牧兽医》
CAS
北大核心
2021年第18期88-90,152,共4页
Heilongjiang Animal Science And veterinary Medicine
关键词
羊
金黄色葡萄球菌
分离
鉴定
耐药性
sheep
Staphylococcus aureus
isolation
identification
drug resistance
