摘要
目的分析ABO正反定型不符的原因,鉴定出正确血型,保证患者用血安全。方法选取2012年1月至2020年12月南京医科大学康达学院附属涟水人民医院住院患者ABO血型正反定型不符标本进行研究。ABO正定型采用微柱凝胶卡手工加样法,反定型采用盐水试管法。对于正反定型不符标本,根据患者临床资料加做相关试验,如增强抗原抗体反应试验、吸收试验、放散试验、不规则抗体筛选试验等。结果2012~2020年本院输血科共鉴定出51例ABO正反定型不符标本,影响因素分别为:抗原减弱14例(27.45%),造血干细胞移植1例(1.96%),冷自身抗体16例(31.37%),抗体减弱11例(21.57%),血型物质过多4例(7.84%),多发性骨髓瘤5例(9.81%)。其中冷自身抗体16例(31.37%),所占比例最多。结论输血科工作人员应熟悉各种实验的操作规程、注意事项及疾病对血型鉴定的影响,会灵活选择、联合运用各实验方法,综合判断血型结果。
Objective To analyze the causes of the discrepancy of ABO positive and negative typing,identify the correct blood type,and ensure the safety of patients.Methods From January 2012 to December 2020 in Lianshui People′s Hospital Affiliated to Kangda College of Nanjing Medical University,ABO blood group positive and negative typing specimens were selected for research.The microcolumn gel card manual sampling method was used for ABO positive typing,and the salt tube method was used for the reverse typing.For samples that did not conform to the positive and negative setting,relevant tests were added based on the patient′s clinical data,such as enhanced antigen antibody reaction test,absorption test,diffusion test,and irregular antibody screening test,etc.Results from 2012 to 2020,a total of 51 cases of ABO positive and negative typing specimens were identified in the blood transfusion department of our hospital.The influencing factors were as follows:antigen weakening in 14 cases(27.45%),hematopoietic stem cell transplantation in 1 case(1.96%),cold autoantibody in 16 cases(31.37%),antibody weakening in 11 cases(21.57%),excessive blood group material in 4 cases(7.84%),and multiple myeloma in 5 cases(9.81%).Sixteen cases(31.37%)cold autoantibodies accounted for the largest proportion among them.Conclusion The staff of blood transfusion department should be familiar with the operating procedures of various experiments,matters needing attention and the influence of diseases on blood group identification,and be able to flexibly select and combine various experimental methods to comprehensively judge the blood group results.
作者
郑兆丽
刘静
程雪梅
ZHENG Zhao-li;LIU Jing;CHENG Xue-mei(Department of Blood Transfusion,Lianshui People′s Hospital Affiliated to Kangda College of Nanjing Medical University,Jiangsu Province,Lianshui 223400,China)
出处
《中国当代医药》
CAS
2021年第29期170-173,共4页
China Modern Medicine
关键词
ABO血型鉴定
正反定型不符
原因分析
解诀方法
ABO blood group identification
Discrepancy of positive and negative stereotypes
Cause analysis
Solutions
