摘要
目的对蓝桉Eucalyptus globulus果实的化学成分进行研究。方法采用硅胶柱色谱、SephadexLH-20凝胶柱色谱及半制备高效液相色谱法分离纯化,通过有机波谱方法鉴定化合物结构,采用MTT法测定部分化合物对人乳腺癌细胞MCF-7的细胞毒活性。结果从蓝桉果实石油醚萃取物中分离得到15个化合物,分别鉴定为大黄酚(1)、eucarobustol E(2)、eucarobustol G(3)、eucalyptal A(4)、eucalyptal C(5)、eucalyptin C(6)、eucalyptin D(7)、eucalyptin E(8)、eucalrobusone A(9)、eucalrobusone C(10)、eucalrobusone F(11)、eucalrobusone U(12)、eucalyptone(13)、euglobal-Ia1(14)、euglobal-Ia2(15)。细胞毒活性结果显示,化合物6、7、10、12、13对MCF-7细胞具有明显细胞毒活性,20μmol/L浓度下抑制率分别为71.83%、71.11%、70.00%、80.53%、79.87%,化合物12对MCF-7细胞显示出较强的细胞毒活性,其半数抑制浓度(IC50)为13.84μmol/L。结论分离得到的成分均为酚性成分,其中化合物1为首次从桉属植物中分离得到,化合物2、3为首次从该植物中分离得到,化合物9~15为首次从蓝桉果实中分离得到;大部分化合物在20μmol/L的浓度下表现出较好的抗MCF-7细胞活性。
Objective To isolate and identify the chemical constituents from the fruits of Eucalyptus globulus.Methods The monomer compounds were separated and purified by silica gel column chromatography,Sephadex LH-20 gel column chromatography and semi-preparative high performance liquid chromatography,and the structure of the compounds was identified by organic spectroscopy.MTT method was used to detect the cytotoxic activity of partial compounds on MCF-7 cells.Results Fifteen compounds were separated from the petroleum ether layer extract of E.globulus,which were identified as chrysophanol(1),eucarobustol E(2),eucarobustol G(3),eucalyptal A(4),eucalyptal C(5),eucalyptin C(6),eucalyptin D(7),eucalyptin E(8),eucalrobusone A(9),eucalrobusone C(10),eucalrobusone F(11),eucalrobusone U(12),eucalyptone(13),euglobal-Ia1(14)and euglobal-Ia2(15).Compounds 6,7,10,12 and 13 had obvious cytotoxic activity on MCF-7 cells with the inhibition rates of 71.83%,71.11%,70.00%,80.53%,and 79.87%,respectively at concentration of 20μmol/L.The compound 12 showed significant cytotoxic activity against MCF-7 with IC50 value of 13.84μmol/L.Conclusion Compounds 1—15 are phenolic constituents.Compounds 1 was isolated from Eucalyptus for the first time,compounds 2 and 3 were isolated from this plant for the first time,and compounds 9—15 were isolated from the fruits of E.globulus for the first time.The cytotoxic activity results showed that most of the compounds showed significant anti-MCF-7 activity at a concentration of 20μmol/L.
作者
于欢
李敏
张旭
王玲莉
王佳
张冬丽
许嵘
唐生安
YU Huan;LI Min;ZHANG Xu;WANG Ling-li;WANG Jia;ZHANG Dong-li;XU Rong;TANG Sheng-an(Tianjin Key Laboratory on Technologies Enabling Development of Clinical Therapeutics and Diagnostics(Theranostics),School of Pharmacy,Tianjin Medical University,Tianjin 300070,China;School of Pharmacy,Quanzhou Medical College,Quanzhou 362011,China)
出处
《中草药》
CAS
CSCD
北大核心
2021年第18期5489-5495,共7页
Chinese Traditional and Herbal Drugs
基金
天津市教委科研计划项目(2019ZD031)。
