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纳豆芽胞杆菌固定化载体改性及吸附研究

Functional Improvements on Immobilization Carrier of Bacillus Culture for Natto Fermentation
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摘要 【目的】以麦饭石为载体,制备高效的纳豆芽胞杆菌固体菌剂,为纳豆芽胞杆菌的规模化应用奠定基础。【方法】运用酸、碱、盐等方法探索麦饭石进行改性条件,扩大麦饭石孔隙度及比表面积,提升其吸附能力;并通过添加保护剂(葡萄糖、糊精、淀粉)增加保藏期固体菌剂的有效活菌数残留率,为长期保藏固体粉剂提供技术支持。【结果】(1)确定了纳豆芽胞杆菌最适培养温度为40℃,最适培养pH为7.5。(2)经氯化钠、盐酸、硫酸和氢氧化钠溶液改性后的麦饭石吸附最大有效活菌数分别为13.17、12.6、12.7和11.9(×10^(8) CFU·g^(-1))。其中0.5 mol·L^(-1)氯化钠溶液改性效果最好,吸附有效活菌数达到13.17(×10^(8) CFU·g^(-1))。(3)对照组、添加保护剂组(2%糊精、2%葡萄糖、2%淀粉)保藏至6个月时,有效活菌数分别为2.91、4.1、5.42和4.63(×10^(8) CFU·g^(-1))。活菌数残留率为初始活菌数的22%、31%、41%和35%,其中2%葡萄糖保护剂比对照组有效活菌数残留率提高了1.86倍。【结论】改性麦饭石能极大提升麦饭石的吸附能力,同时保护剂的添加有利于麦饭石固体芽胞杆菌粉剂的长期保藏,本研究为麦饭石在微生物菌剂制备工艺的应用提供了一定的技术支持。 【Objective】The physical properties of maifanshi,a volcanic rock,were modified to improve its porosity and adsorption to be used as an immobilization carrier for natto fermentation bacteria that was further added with protective agents to extend the shelf life of viable Bacillus natto.【Method】Maifanshi was treated by acid,alkali,or salt to modify the porosity and surface adsorption property as a microbial carrier.Various protective agents,such as glucose,dextrin,and/or starch were incorporated to help prolong the viability of the imbedded B.natto powder.【Result】(1)The optimum conditions for B.natto culture included 40℃and pH 7.5.(2)The maximum viable bacteria count was the highest at 13.17×10^(8) CFU·g^(-1) on the maifanshi carriers modified by 0.5 mol·L^(-1) sodium chloride solution.It was followed by a count of 12.7×10^(8) CFU·g^(-1) on maifanshi treated by sulfuric acid,12.6×10^(8) CFU·g^(-1) on maifanshi treated by hydrochloric acid,and 11.9×10^(8) CFU·g^(-1) on maifanshi treated by sodium hydroxide.(3)The viable B.natto counts after 6 months in storage on control was 2.91×10^(8) CFU·g^(-1) as compared to those immobilized on the carriers with addition of 2% dextrin,2% glucose,or 2%starch being 4.1×10^(8) CFU·g^(-1),5.42×10^(8) CFU·g^(-1),and 4.63×10^(8) CFU·g^(-1),respectively.The retention of viable B.natto on the NaCltreated maifanshi with the addition of dextrin was 31%,that of glucose 41%,that of starch 35%,and that of control 22%.【Conclusion】As a bacteria carrier,maifanshi could be significantly further improved on the adsorption capacity by modification such as NaCl treatment.With an addition of 2% glucose on the immobilized system,the retained viable B.natto count could rise 1.86 times over control in 6 months of storage.It appeared that this study had paved the way of developing a practical application of maifanshi for natto fermentation industry.
作者 陈龙军 林程 林陈强 张慧 贾宪波 方宇 陈济琛 CHEN Longjun;LIN Cheng;LIN Chenqiang;ZHANG Hui;JIA Xianbo;FANG Yu;CHEN Jichen(Soil and Fertilizer Institute,Fujian Academy of Agricultural Sciences,Fuzhou,Fujian 350003,China;Fuzhou Agricultural and Rural Bureau,Fuzhou,Fujian 350003,China)
出处 《福建农业学报》 CAS CSCD 北大核心 2021年第7期855-860,共6页 Fujian Journal of Agricultural Sciences
基金 福建省农业科学院探索创新项目(AA2018-13) 福建省科技计划区域发展项目(2019Y3005) 福建省科技计划公益类专项(2018R1022-7)、(2019R1025-4) 国家重点研发计划项目(2018YFD0200706)。
关键词 纳豆芽胞杆 麦饭石 改性 保护剂 吸附 Bacillus natto maifanshi modification protectant adsorption
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