摘要
目的本研究拟通过艾地苯醌对血小板生物学特性的影响,以期探讨其在血小板保存方面的应用价值。方法体外实验中,富小板血浆加入终浓度为10μg/mL艾地苯醌作为实验组,检测血小板粘附和聚集功能,血浆丙二醛、还原型谷胱甘肽、以及血小板膜表面CD62p和线粒体膜电位的变化。结果与对照组相比,实验组血小板的粘附率和聚集率均有一定程度的下降(P<0.05);血浆丙二醛水平降低(P<0.05)而谷胱甘肽水平升高(P<0.05);CD62p的阳性率无显著性变化,但JC-1的阳性率有显著性增加(P<0.05)。结论10μg/mL艾地苯醌有利于血小板线粒体的代谢,但是对血小板的粘附和聚集功能产生一定的抑制作用。
Objective To explore the application value of idebenone in platelet preservation through its effect of on platelet biological characteristics.Methods In vitro experiment,platelet-rich plasma was added idebenone with the final concentration of 10μg/mL as the experimental group.We detected the platelet adhesion and aggregation function,plasma malondialdehyde(MDA)concentration,reduced glutathione(GSH)concentration,and CD62p expression on platelet membrane surface and JC-1 on mitochondrion surface.Results Compared with the control group,platelet adhesion and aggregation rate decreased to a certain extent(P<0.05);The MDA level decreased(P<0.05)while reduced GSH level increased(P<0.05);The positive rate of CD62p showed no significant change,but the positive rate of JC-1 increased significantly(P<0.05).Conclusion Idebenone would be beneficial to the metabolism of platelet mitochondria,but it has a certain inhibitory effect on platelet adhesion and aggregation with the concentration of 10μg/mL.
作者
林俊填
麦志周
杨富燕
伍伟健
刘运芝
余玉婷
LIN Jun-tian;MAI Zhi-zhou;YANG Fu-yan(Foshan Central Blood Station,528000)
出处
《临床输血与检验》
CAS
2021年第5期628-631,共4页
Journal of Clinical Transfusion and Laboratory Medicine
基金
广东省佛山市自筹经费类科技计划项目(No.1920001000780)资助。
