摘要
目的:探讨阿尔茨海默病(AD)患者血清微小RNA(miRNA)-137、三磷酸腺苷结合盒转运蛋白7(ABCA7)水平变化及临床意义。方法:选取我院2019年1月至2020年12月收治的138例AD患者为AD组,根据临床痴呆评定量表(CDR)分为轻度组(n=48)、中度组(n=59)、重度组(n=31),另选取同期64名体检健康者为对照组。比较两组血清miR-137、ABCA7、β-淀粉样蛋白42(Aβ42)、Aβ40、Aβ42/Aβ40、血脂水平和简易精神状态量表(MMSE)评分。Spearman相关性分析AD组血清miR-137、ABCA7水平与血清Aβ42、Aβ40、Aβ42/Aβ40、血脂水平和MMSE评分的相关性。ROC曲线分析血清miR-137、ABCA7水平对AD的诊断价值。结果:AD组血清miR-137、Aβ42、高密度脂蛋白胆固醇(HDL-C)水平和Aβ42/Aβ40、MMSE评分明显低于对照组,ABCA7、Aβ40、总胆固醇(total cholesterol,TC)、低密度脂蛋白胆固醇(LDL-C)水平明显高于对照组(P<0.05)。Spearman相关性分析显示,AD组血清miR-137水平与Aβ42、Aβ42/Aβ40、HDL-C和MMSE评分呈正相关,与Aβ40、TC、LDL-C水平、CDR评分呈负相关(P<0.05);血清ABCA7水平与Aβ42、Aβ42/Aβ40、HDL-C和MMSE评分呈负相关,与Aβ40、TC、LDL-C水平、CDR评分呈正相关(P<0.05)。ROC曲线显示,miR-137+ABCA7诊断AD的曲线下面积(AUC=0.925)明显大于miR-137(AUC=0.837)、ABCA7(AUC=0.823)单独诊断(P<0.05),敏感度、特异度为85.51%、84.37%。结论:AD患者血清miR-137水平明显降低,ABCA7水平明显提高,与AD患者Aβ沉积、胆固醇代谢异常、病情密切相关,可能成为AD诊断、病情严重程度评估标志物,二者联合检测价值更高。
Objective:To investigate the changes and clinical significance of serum microRNA(miRNA)-137 and adenosine triphosphate binding cassette transporter 7(ABCA7)levels in patients with Alzheimer's disease(AD).Methods:A total of 138 AD patients admitted to our hospital from January 2018 to December 2019 were selected as the AD group.According to the clinical dementia rating(CDR)scale,they were divided into mild group(n=48)and moderate group(n=59),severe group(n=31),and 64 healthy people during physical examination during the same period were selected as the control group.Spearman correlation analysis was conducted to analyze the correlation between AD group's serum miR-137,ABCA7 levels and serum Aβ42,Aβ40,Aβ42/Aβ40,blood lipid levels and MMSE score.ROC curve was plotted to analyze serum miR-137 and ABCA7 levels in the diagnosis of AD.Results:The serum miR-137,Aβ42,high-density lipoprotein cholesterol(HDL-C)levels,Aβ42/Aβ40 and MMSE scores in the AD group were significantly lower than those in the control group,and ABCA7,Aβ40,total cholesterol(TC),and low-density lipoprotein cholesterol(LDL-C)levels were significantly higher than those of the control group(P<0.05).Spearman correlation analysis showed that serum miR-137 levels in AD group were positively correlated with Aβ42,Aβ42/Aβ40,HDL-C and MMSE scores,and negatively correlated with Aβ40,TC,LDL-C levels,and CDR scale(P<0.05);Serum ABCA7 levels were negatively correlated with Aβ42,Aβ42/Aβ40,HDL-C and MMSE scores,and positively correlated with Aβ40,TC,LDL-C levels,and CDR scale(P<0.05).The ROC curve showed that the area under the curve(AUC=0.925)of miR-137+ABCA7 for the diagnosis of AD was significantly greater than that of miR-137(AUC=0.837),ABCA7(AUC=0.823)in diagnosed alone(P<0.05),and the sensitivity and specificity were 85.51%and 84.37%.Conclusion:The level of serum miR-137 in AD patients is significantly reduced,and the level of ABCA7 is significantly increased.It is closely related to Aβdeposition,abnormal cholesterol metabolism,and disease cond
作者
周育蕾
陈艳洁
刘泽民
李艳琴
段玉香
张凤莲
ZHOU Yulei;CHEN Yanjie;LIU Zemin(Tangshan People's Hospital , Hebei Tangshan 06300, China)
出处
《河北医学》
CAS
2021年第10期1652-1657,共6页
Hebei Medicine
基金
河北省医学科研课题,(编号:201801158)。
